Korean J Anat.  2006 Apr;39(2):129-138.

Morphological Study for Osteoinduction by Human Fibroblasts Transduced with rhBMP-7 Adenovirus

• Park JK
• Sung EG
• Kim JY
• Kim JR
• Ahn MH
• Kim HT
• Moon YS
• Song IH

Abstract

To evaluate availability of the BMP-7 adenovirus (AdBMP-7) as a gene therapy for osteoinduction, we investigated in morphological aspect at 1, 2, 4, 6 weeks after cells injection. Primary cultured human dermal fibroblasts, transduced with AdBMP-7, were injected into gastrocnemius muscle of the nude mice. One week after fibroblasts transplantation new tissue was observed in the muscle. Majority of new tissue was evaluated as cartilage and calcification in the matrix was confirmed by Von Kossa stain as well as electron microscopy. Two weeks after transplantation, spongy bone was built up and adipocytes were observed in intertrabecular spaces. Osteoblasts and osteoclasts were observed in the bony tissue surface. In the result of Von Kossa-Van Gieson stain, osteolysis was dominant in bony trabeculae. Bone marrow was established in 4th weeks with intertrabecular space filled up by hematopoietic cells. At the 6th weeks, the number of trabeculae decreased and thickness of the cortical bone was increased. A great part of bone matrix has laminar structure which run paralleled to surface and which included osteocytes and canaliculi. These data demonstrate that cell mediated AdBMP-7 for gene therapy initiate development of cartilage and calcification of matrix within 1 week and complete bone and bone marrow formation within 4 weeks, so then, could be made practical application for promotion of osteoinduction.

Keyword

Osteoinduction; BMP-7 adenovirus; Gene therapy; Fibroblast

MeSH Terms

Adenoviridae*
Adipocytes
Animals
Bone Marrow
Bone Matrix
Bone Morphogenetic Protein 7
Cartilage
Fibroblasts*
Genes, vif
Genetic Therapy
Humans*
Mice
Mice, Nude
Microscopy, Electron
Muscle, Skeletal
Osteoblasts
Osteoclasts
Osteocytes
Osteolysis
Bone Morphogenetic Protein 7