J Korean Acad Periodontol.  2002 Jun;32(2):389-401. 10.5051/jkape.2002.32.2.389.

The effect of rhBMP-2 on the osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts in vitro

• Kim HJ
• Choi SM
• Ku Y
• Rhyu IC
• Chung CP
• Han SB
• Kim KH
• Lee YM

• KMID: 2057050
• DOI: http://doi.org/10.5051/jkape.2002.32.2.389

Abstract

BMP can induce ectopic bone formation when implanted into sites such as rat muscle and can greatly enhance healing of bony defects when applied exogenously. In addition, BMP stimulated osteoblastic differentiation in vitro in various types of cells. The aim of this study was to investigate the effect of recombinant human bone morphogenetic protein(rhBMP-2) on the proliferation and osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts. The cell number and alkaline phosphatase activity were measured in 3 experimental groups of human periodontal ligament cells and gingival fibroblasts(control group, rhBMP-2 50ng/ml group, and rhBMP-2 100ng/ml group) at 1 and 2 weeks after culture. At the same time, total RNA of cultured cells were extracted and reverse trascription polymerase chain reaction(RT-PCR) was performed to determine the expression of mRNA of bone matrix protein. RhBMP-2 had no effect on the cell proliferation of human periodontal ligament cells and gingival fibroblasts. Alkaline phosphatase activity was elevated significantly by rhBMP-2 in both cells. And periodontal ligament cells showed significantly higher alkaline phosphatase activity than gingival fibroblasts. beta-actin, type I collagen, alkaline phosphatase, BMP-2 mRNA were expressed in all of the samples. Osteopontin, osteocalcin mRNA were expressed in all periodontal ligament cell groups, and rhBMP-2 50ng/ml group, rhBMP-2 100ng/ml group of 2 week culture period of gingival fibroblasts. Bone sialoprotein mRNA was only expressed in rhBMP-2 50ng/ml group and rhBMP-2 100ng/ml group of 2-week culture period. These results suggest that rhBMP-2 stimulates osteoblastic differentiation in human periodontal ligament cells and gingival fibroblasts in vitro.

Keyword

recombinant human bone morphogenetic protein-2(rhBMP-2); periodontal ligament cell; osteoblast; differentiation

MeSH Terms

Actins
Alkaline Phosphatase
Animals
Bone Matrix
Cell Count
Cell Proliferation
Cells, Cultured
Collagen Type I
Fibroblasts*
Humans*
Integrin-Binding Sialoprotein
Osteoblasts*
Osteocalcin
Osteogenesis
Osteopontin
Periodontal Ligament*
Rats
RNA
RNA, Messenger
Actins
Alkaline Phosphatase
Collagen Type I
Integrin-Binding Sialoprotein
Osteocalcin
Osteopontin
RNA
RNA, Messenger