Maxillofac Plast Reconstr Surg.  2014 May;36(3):94-102.

Bone Healing Properties of Autoclaved Autogenous Bone Grafts Incorporating Recombinant Human Bone Morphogenetic Protein-2 and Comparison of Two Delivery Systems in a Segmental Rabbit Radius Defect

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Korea. kimoms@yuhs.ac
  • 2Department of Oral and Maxillofacial Surgery, Wonkwang University School of Dentistry, Korea.
  • 3Department of Oral and Maxillofacial Surgery, National Health Insurance Service Ilsan Hospital, Korea.
  • 4Department of Oral Biology, Yonsei University College of Dentistry, Korea.
  • 5Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Korea.

Abstract

PURPOSE
This study aims to validate the effect of autoclaved autogenous bone (AAB), incorporating Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), on critical-sized, segmental radius defects in rabbits. Delivery systems using absorbable collagen sponge (ACS) and fibrin glue (FG) were also evaluated.
METHODS
Radius defects were made in 12 New Zealand white rabbits. After autoclaving, the resected bone was reinserted and fixed. The animals were classified into three groups: only AAB reinserted (group 1, control), and AAB and ErhBMP-2 inserted using an ACS (group 2) or FG (group 3) as a carrier. Animals were sacrificed six or 12 weeks after surgery. Specimens were evaluated using radiology and histology.
RESULTS
Micro-computed tomography images showed the best bony union in group 2 at six and 12 weeks after operation. Quantitative analysis showed all indices except trabecular thickness were the highest in group 2 and the lowest in group 1 at twelve weeks. Histologic results showed the greatest bony union between AAB and radial bone at twelve weeks, indicating the highest degree of engraftment.
CONCLUSION
ErhBMP-2 increases bony healing when applied on AAB graft sites. In addition, the ACS was reconfirmed as a useful delivery system for ErhBMP-2.

Keyword

Bone morphogenetic protein 2; Autogenous autoclaved bone; Critical bone defect; Rabbit radius; BMP carrier

MeSH Terms

Animals
Bone Morphogenetic Protein 2
Collagen
Escherichia
Fibrin Tissue Adhesive
Humans
Porifera
Rabbits
Radius*
Transplants*
Bone Morphogenetic Protein 2
Collagen
Fibrin Tissue Adhesive
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