Yonsei Med J.  1992 Mar;33(1):54-63. 10.3349/ymj.1992.33.1.54.

Effect of porcine bone morphogenetic protein on healing of bone defect in the rabbit radius

• Kim NH
• Yang KH
• Lee HM
• Oh SH

• KMID: 1117765
• DOI: http://doi.org/10.3349/ymj.1992.33.1.54

Abstract

Segmental long bone defects due to infection or trauma is a difficult problem to manage in patients. We studied the effect of porcine bone morphogenetic protein (pBMP) on healing of defects in the rabbit radius. Porcine BMP was separated and purified from the tibia and femur of pigs by repeated solubilization and precipitation of the protein with different concentrations of urea and GuHCl. The osteoinductive activity of pBMP was confirmed by bioassay using No. 615 mice. In rabbits, about a 15 mm length of radii were removed and 20 mg of pBMP was implanted in the defected area with fibrin sealant (FS), while only FS was implanted in controls. Union of the affected area was observed in 6 weeks in the experimental side. There was no definite evidence of bone bridging across the affected area in the controls. This suggests that pBMP has a bone forming activity in other species and the clinical use of pBMP in treating patients with segmental bone defects is promising.

Keyword

Bone morphogenetic protein; porcine; purification; bone defect; radius; rabbit

MeSH Terms

Animal
Bone Morphogenetic Proteins
Growth Substances/*pharmacology
Osteogenesis/*drug effects
Proteins/*pharmacology
Rabbits
Radius/drug effects/pathology/radiography
Support, Non-U.S. Gov't
Swine