J Korean Acad Nurs.  2014 Aug;44(4):371-380. 10.4040/jkan.2014.44.4.371.

Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats

• Kim JI
• Choe MA

• KMID: 1791866
• DOI: http://doi.org/10.4040/jkan.2014.44.4.371

Abstract

PURPOSE
The purpose of this study was to examine the effects of Cu/Zn SOD on reduction of hindlimb muscular atrophy induced by cisplatin in rats.
METHODS
Forty-two rats were assigned to three groups; control group, Cisplatin (CDDP) group and cisplatin with Cu/Zn SOD (CDDP-SOD) group. At day 35 hindlimb muscles were dissected. Food intake, activity, withdrawal threshold, muscle weight, and Type I, II fiber cross-sectional area (CSA) of dissected muscles were measured. Relative SOD activity and expression of MHC and phosphorylated Akt, ERK were measured after dissection.
RESULTS
Muscle weight and Type I, II fiber CSA of hindlimb muscles in the CDDP group were significantly less than the control group. Muscle weight and Type I, II fiber CSA of hindlimb muscles, food intake, activity, and withdrawal thresholds of the CDDP-SOD group were significantly greater than the CDDP group. There were no significant differences in relative SOD activities of hindlimb muscles between the CDDP-SOD and CDDP groups. MHC expression and phosphorylated Akt, ERK of hindlimb muscles in the CDDP-SOD group were significantly greater than the CDDP group.
CONCLUSION
Cu/Zn SOD attenuates hindlimb muscular atrophy induced by cisplatin through increased food intake and activity. Increment of phosphorylated Akt, ERK may relate to attenuation of hindlimb muscular atrophy.

Keyword

Antioxidant; Cisplatin; Rat; Muscular atrophy; Superoxide dismutase

MeSH Terms

Animals
Body Weight/drug effects
Cisplatin/*toxicity
Disease Models, Animal
Extracellular Signal-Regulated MAP Kinases/metabolism
Hindlimb
Male
Muscle, Skeletal/*drug effects/enzymology/metabolism
Muscular Atrophy/*chemically induced/metabolism/pathology
Phosphorylation
Proto-Oncogene Proteins c-akt/metabolism
Rats
Rats, Sprague-Dawley
Recombinant Proteins/biosynthesis/genetics/pharmacology
Superoxide Dismutase/genetics/metabolism/pharmacology
Superoxides/metabolism
Cisplatin
Extracellular Signal-Regulated MAP Kinases
Proto-Oncogene Proteins c-akt
Recombinant Proteins
Superoxide Dismutase
Superoxides

Figure

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  Figure 1 Relative SOD activity of three groups.

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  Figure 2 A. Expression of phosphorylated Akt, phosphorylated ERK and expression of MHC of soleus in C, CDDP and CDDP-SOD rats. B. Expression of phosphorylated Akt, phosphorylated ERK and expression of MHC of plantaris in C, CDDP and CDDP-SOD rats.

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