Korean J Vet Res.  2013 Dec;53(4):217-224.

Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice

• Ahn DJ
• Youm JW
• Kim SW
• Yoon WK
• Kim HC
• Hur TY
• Joung YH
• Jeon JH
• Kim HS

Abstract

Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-S0.7) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-S0.7 gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-S0.7 protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-S0.7 antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.

Keyword

oral administration; potato-derived antigen; transmissible gastroenteritis virus; transgenic plant

MeSH Terms

Administration, Oral
Animals
Blotting, Northern
Blotting, Western
Coronavirus
DNA
Enzyme-Linked Immunosorbent Assay
Escherichia
Gastroenteritis
Glycoproteins*
Immunoglobulin A
Immunoglobulins
Mice*
Plants, Genetically Modified
Polymerase Chain Reaction
RNA, Messenger
Solanum tuberosum*
Transmissible gastroenteritis virus*
Vaccines
DNA
Glycoproteins
Immunoglobulin A
Immunoglobulins
RNA, Messenger
Vaccines