J Korean Soc Hypertens.  2011 Sep;17(3):95-102. 10.5646/jksh.2011.17.3.95.

The Preventive and Therapeutic Effects of Aronox Extract on Metabolic Abnormality and Hypertension

• Park YM
• Park JB

• KMID: 1750387
• DOI: http://doi.org/10.5646/jksh.2011.17.3.95

Abstract

BACKGROUND
Aronox is an anthocyanin-rich extract from Aronia melanocarpa E which is known to have anti-inflammatory effect in atherosclerosis due to high anti-oxidative activity. This study was conducted to evaluate the preventive and therapeutic effect of Aronox on hypertension and metabolic status in spontaneously hypertensive rats (SHR).
METHODS
Seven-week-old male SHR were orally administrated with Aronox (low dose; 100 mg/kg, n = 4 or high dose; 200 mg/kg, n = 3) or amlodipine (10 mg/kg, n = 7) and sham (n = 10) for 8 weeks. Aronox was administered 30 mg/kg for the first 3 weeks and then increased to 100 mg/kg (low dose group) or 100 mg/kg to 200 mg/kg (high dose group). Systolic blood pressure (SBP) was measured every week by tail cuff method. At 8 weeks, fasting lipid level was measured. Heart and kidney stained with Masson's trichrome.
RESULTS
Aronox or amlodipine treatment showed significantly lower SBP compared with sham (202.2 +/- 10.2 mm Hg in low dose group, 202.0 +/- 12.6 mm Hg in high dose group and 187.4 +/- 22.7 mm Hg in amlodipine group vs. 224.4 +/- 12 mm Hg in sham-SHR, p < 0.005). There were no significant differences in cardiac and renal weight corrected by body weight among 3 groups. Aronox and amlodipine treatments significantly decreased fasting glucose and showed a trend of decrease in triglyceride level. Aronox or amlodipine treatment for 8 weeks showed less collagen deposition changes compared to sham.
CONCLUSIONS
Aronox showed significant antihypertensive effects, decreased fasting glucose, and less cardiac and renal fibrosis in SHR. These results suggest that Aronox can be used as an adjuvant therapy or functional food in hypertension.

Keyword

Aronia melanocarpa; Hypertension; Glucose; Spontaneously hypertensive rats

MeSH Terms

Amlodipine
Atherosclerosis
Blood Pressure
Body Weight
Collagen
Fasting
Fibrosis
Functional Food
Glucose
Heart
Humans
Hypertension
Kidney
Male
Photinia
Rats, Inbred SHR
Salicylamides
Tail
Amlodipine
Collagen
Glucose
Salicylamides

Figure

• 

  Fig. 1. The flow chart of systolic blood pressure (SBP) changes during treatment of aronox or amlodpine. From the point of 5 week treatment, the treatment groups with aronox or amlodipine showed significant lower SBP compared with sham group. *p < 0.05 for sham vs. aronox 100 mg/kg, 200 mg/kg and amlodipine. SHRs, spontaneously hypertensive rats.

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  Fig. 2. Microscopic findings of effects of aronox or amlodpine on cardiac fibrosis. Masson’s trichrome staining of left ventricle for collagen (blue) detection showed more fibrotic changes around perivascular region in sham group (A) compared with aronox or amlodipine treatment group (B-D) (100×). SHRs, spontaneously hypertensive rats.

• 

  Fig. 3. Microscopic findings of effects of aronox or amlodpine on renal fibrosis. Masson’s trichrome staining of kidney for collagen (blue) detection showed more fibrotic changes around perivascular (100×) region in sham group (A) compared witharonox or amlodipine treatment group (B-D) (100×). SHRs, spontaneously hypertensive rats.

Reference

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