J Dent Rehabil Appl Sci.  2021 Sep;37(3):123-129. 10.14368/jdras.2021.37.3.123.

Preventive effects of shiitake mushroom extract on candida stomatitis

• Yoo HJ

• KMID: 2525986
• DOI: http://doi.org/10.14368/jdras.2021.37.3.123

Abstract

Purpose
The purpose of this study was to investigate antifungal activity of shiitake mushroom yeast and hyphal type of Candida albicans.
Materials and Methods
The extract from shiitake mushroom was collected by drying the supernatant after soaking shiitake mushrooms in water or ethanol. The antifungal activity of the extracts against yeast type of C. albicans was investigated by the susceptibility assay using microplate. C. albicans biofilm was formed on 12-well plate using Ham’s F-12 medium in CO 2 incubator and treated with the ethanol extract. Furthermore, C. albicans biofilm was formed on denture base resin disk and treated with or without the ethanol extract in the presence of denture cleanser. Live C. albicans in biofilm was counted by cultured colony forming unit value after inoculated on agar plate.
Results
Ethanol extract from shiitake mushroom showed stronger antifungal activity against yeast type of C. albicans compared to its water extract. The ethanol extract significantly reduced count of C. albicans in hyphal biofilm (P < 0.05). Also, the ethanol extract showed synergistically antifungal effect with denture cleanser on candidal biofilm on denture base resin disk (P < 0.05).
Conclusion
The ethanol extract of shiitake mushroom may be a candidate for preventing candidal stomatitis as well as denture-related stomatitis.

Keyword

C. albicans; candidal stomatitis; extract of shiitake mushroom

Figure

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  Fig. 1 The antifungal activity of extract from shiitake mushroom on yeast type of C. albicans. C. albicans was cultivated with or without water or ethanol extract from shiitake in the various concentration. The growth of C. albicans was measured by a spectrophometer at 660 nm of wavelength. *symbol indicates significant difference compared to control group (P < 0.05).

• 

  Fig. 2 Effect of the ethanol extract of candidal biofilm. C. albicans was formed biofilm on 12-well plate and treated with the ethanol extract for 1 min. After disrupting biofilm mechanically, C. albicans in the biofilm was resuspended with TSB and inoculated TSA plate. The plate was incubated, and the colonies on the agar plates were counted. *symbol indicates significant difference compared to control group (P < 0.05).

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  Fig. 3 Synergistic effect of the ethanol extract on reaction of denture cleanser for candidal biofilm on denture base resin. C. albicans was formed biofilm on denture base resin disk. The prepared disk was soaked in denture cleanser solution in the presence or the absence of the ethanol extract. After disrupting biofilm mechanically, C. albicans in the biofilm was resuspended with TSB and inoculated TSA plate. The plate was incubated, and the colonies on the agar plates were counted. *symbol indicates significant difference compared to control group (P < 0.05).

Reference

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