J Dent Rehabil Appl Sci.  2022 Jun;38(2):90-96. 10.14368/jdras.2022.38.2.90.

Effects of Shiitake mushroom extract on antimicrobial activity against periodontopathogens and inflammatory condition of human gingival fibroblast

• Jeon YM

• KMID: 2535016
• DOI: http://doi.org/10.14368/jdras.2022.38.2.90

Abstract

Purpose
The purpose of this study was to investigate antimicrobial activity of extracts from shiitake mushroom against periodontopathogens and its cytotoxicity for human gingival fibroblast.
Materials and Methods
Shiitake mushroom was soaked in water and acetone, and the supernatant was dried to collect its extract. The susceptibility of periodontopathogens for the extracts was investigated. Human gingival fibroblast was treated with the extracts, and the cell viability was measured CCK-8 solution.
Results
The water extract from shiitake mushroom significantly reduced the growth of periodontopathogens at 2.5 mg/ml (P < 0.05). The acetone extract significantly inhibited the growth of Porphyromonas gingivalis and Tannerella forsythia at 0.32 mg/ml and Treponema denticola growth at 0.64 mg/ml (P < 0.05). The cytotoxicity of the extract was shown at a concentration of 2.5 mg/ml. The extracts with a concentration of 1.25 mg/ml appeared to be reduce cell viability after 4 h.
Conclusion
The extracts of shiitake mushroom have antimicrobial activity against periodontitis-causing bacteria and relieving inflammation. Therefore, the extracts may be a candidate for preventing and treating periodontal disease.

Keyword

antimicrobial activity; extract of shiitake mushroom; periodondopathogen

Figure

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  Fig. 1 The susceptibility of P. gingivalis for the extracts from shiitake mushroom. P. gingivalis was incubated with or without the extracts in the various concentrations. The growth of P. gingivalis was measured by a spectrophotometer at 660 nm wavelength. Asterix (*) indicates significant difference compared with control (P < 0.05). O.D, optical density.

• 

  Fig. 2 The antimicrobial activity of the extracts from shiitake mushroom against T. forsythia. T. forsythia was cultured with or without the extracts in the various concentrations. The growth of T. forsythia was measured by a spectrophotometer at 660 nm wavelength. Asterix (*) indicates significant difference compared with control (P < 0.05). O.D, optical density.

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  Fig. 3 The susceptibility of T. denticola for the extract from shiitake mushroom. T. denticola was incubated with or without the extracts in the various concentrations. The growth of T. denticola was measured by a spectrophotometer at 660 nm wavelength. Asterix (*) indicates significant difference compared with control (P < 0.05). O.D, optical density.

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  Fig. 4 The effect of the extracts on cell viability. HGF-1 treated with the extracts in the various concentrations for 12, and the cell viability was measured by CCK-8. Asterix (*) indicates significant difference compared with control (P < 0.05).

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  Fig. 5 The cytotoxicity of the extracts over time. HGF-1 treated with the extracts at a concentration of 1.25 mg/ml for the various time, and the cell viability was measured by CCK-8. Asterix (*) indicates significant difference compared with control (P < 0.05). O.D, optical density.

Reference

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