Mycobiology.  2008 Mar;36(1):74-76. 10.4489/MYCO.2008.36.1.074.

Extracellular Enzyme Activities of the Monokaryotic Strains Generated from Basidiospores of Shiitake Mushroom

• Kwon HW
• Back IJ
• Ko HG
• You CH
• Kim SH

• KMID: 2052893
• DOI: http://doi.org/10.4489/MYCO.2008.36.1.074

Abstract

To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, beta-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.

Keyword

Basidiospores; Chromogenic media; Extracellular enzyme activity; Shiitake mushroom

MeSH Terms

Amylases
beta-Glucosidase
Cellulases
Humans
Parents
Polygalacturonase
Shiitake Mushrooms
Amylases
Cellulases
Polygalacturonase
beta-Glucosidase