Int J Oral Biol.  2015 Jun;40(2):79-83. 10.11620/IJOB.2015.40.2.079.

Antimicrobial Effect of Coptidis rhizome Extract against Mutans Streptococci and Periodontopathogens

• Park SN
• Lim YK
• Kook JK

• KMID: 1827456
• DOI: http://doi.org/10.11620/IJOB.2015.40.2.079

Abstract

The purpose of the study was to investigate the antimicrobial activity of the methanol extract of Coptidis rhizome against the type strains of cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus, and the periodontopathogens, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extract and the methanol extract fractions of Coptidis rhizome separated by silica gel chromatography were evaluated by determining the minimal bactericidal concentration (MBC) values, using the microdilution method. The cell viability test of the extracts of Coptidis rhizome on the KB cells was also studied by methyl thiazolyl tetrazolium (MTT) assay. Our results showed that the 11th fraction (F11) of the methanol extract had the greatest antimicrobial activity against the tested bacteria, with no associated cytotoxicity on the KB cells, upto a concentration of 50 microg/ml. These results suggest that the silica gel chromatography fraction F11 of the methanol extract of Coptidis rhizome, could be useful in the development of oral hygiene products as an antimicrobial agent for the prevention of dental caries and periodontal diseases.

Keyword

antimicrobial effect; Coptidis rhizome; mutans streptococci; periodontopathogens

MeSH Terms

Aggregatibacter actinomycetemcomitans
Bacteria
Cell Survival
Chromatography
Dental Caries
Humans
KB Cells
Methanol
Oral Hygiene
Periodontal Diseases
Porphyromonas gingivalis
Prevotella intermedia
Rhizome*
Silica Gel
Streptococcus mutans
Streptococcus sobrinus
Treponema denticola
Methanol
Silica Gel