Korean J Parasitol.  2011 Dec;49(4):373-380. 10.3347/kjp.2011.49.4.373.

A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice

• Park HK
• Cho MK
• Park MK
• Kang SA
• Kim YS
• Kim KU
• Lee MK
• Ock MS
• Cha HJ
• Yu HS

• KMID: 1715742
• DOI: http://doi.org/10.3347/kjp.2011.49.4.373

Abstract

We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-alpha (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.

Keyword

Anisakis simplex; As22U; allergic airway inflammation; excretory secretory protein

MeSH Terms

Administration, Intranasal
Animals
Anisakiasis/*immunology/parasitology
Anisakis/*immunology/metabolism
Bronchoalveolar Lavage Fluid
Chemokines/metabolism
Cytokines/analysis/*metabolism
Eosinophils/metabolism
Female
Gene Expression Regulation/*immunology
Helminth Proteins/*immunology
Hypersensitivity/*immunology/parasitology
Immunoglobulin E/immunology
Immunoglobulin G/immunology
Larva/immunology/metabolism
Lung/metabolism
Mice
Mice, Inbred C57BL
Recombinant Proteins/immunology
Th17 Cells/metabolism
Th2 Cells/metabolism
Chemokines
Cytokines
Helminth Proteins
Immunoglobulin E
Immunoglobulin G
Recombinant Proteins