- A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice
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Mun HS, Norose K, Aosai F, Chen M, Yano A
- KMID: 1715245
- Korean J Parasitol.
- 2000 Jun;38(2):107-110.
- doi: 10.3347/kjp.2000.38.2.107
We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g.HSP70-NH2-terminal region, or rT.g.HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts... -
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- Molecular Approach to Allergy Diagnosis and Therapy
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Ferreira F, Wolf M, Wallner M
- KMID: 2130806
- Yonsei Med J.
- 2014 Jul;55(4):839-852.
- doi: 10.3349/ymj.2014.55.4.839
Presently, allergy diagnosis and therapy procedures are undergoing a transition phase in which allergen extracts are being step-by-step replaced by molecule-based products. The new developments will allow clinicians to obtain... -
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- Antibody Response of Mouse Splenocytes Using Mixture of Supernatants of Thymocytes, Adherent and Non-adherent Splenocytes: In-vitro Immunization-II
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Kim D, Noh GW, Kim DH, Kwon OH
- KMID: 1421475
- J Korean Med Sci.
- 1990 Mar;5(1):25-31.
- doi: 10.3346/jkms.1990.5.1.25
We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported... -
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- Expression of a recombinant branched chain alpha-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera
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Lee SM, Ko SH, Park JE, Cha SH
- KMID: 754990
- Exp Mol Med.
- 1998 Jun;30(2):65-71.
Preparation of a pure autoantigen by way of recombinant DNA technology has an important value in an accurate diagnosis or prognosis of an autoimmune disease. BCOADC-E2 subunit, a mitochondrial protein, has been... -
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- Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation
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You MJ
- KMID: 1106074
- J Vet Sci.
- 2005 Mar;6(1):47-51.
The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa... -
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- Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
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Liu YY, Yang WT, Shi SH, Li YJ, Zhao L, Shi CW, Zhou FY, Jiang YL, Hu JT, Gu W, Yang GL, Wang CF
- KMID: 2412568
- J Vet Sci.
- 2017 Jun;18(2):159-167.
- doi: 10.4142/jvs.2017.18.2.159
Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection.... -
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- Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection
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Cho B, Jeon BY, Kim J, Noh J, Kim J, Park M, Park S
- KMID: 2158202
- Yonsei Med J.
- 2008 Oct;49(5):828-835.
- doi: 10.3349/ymj.2008.49.5.828
PURPOSE: Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic... -
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- Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients
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Ahn HJ, Kim S, Nam HW
- KMID: 1080959
- Korean J Parasitol.
- 2003 Jun;41(2):89-96.
- doi: 10.3347/kjp.2003.41.2.89
Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The... -
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- Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
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Lee HK, Lee BH, Seok SH, Baek MW, Lee HY, Kim DJ, Na YR, Noh KJ, Park SH, Kumar DN, Kariwa H, Nakauchi M, Heo SJ, Park JH
- KMID: 1110865
- J Vet Sci.
- 2010 Jun;11(2):165-167.
- doi: 10.4142/jvs.2010.11.2.165
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative... -
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- Development of two novel nontoxic mutants of Escherichia coli heat-labile enterotoxin
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Park EJ, Chang JH, Kim JS, Chung SI, Yum JS
- KMID: 1029939
- Exp Mol Med.
- 1999 Jun;31(2):101-107.
Escherichia coli heat-labile enterotoxin (LT) is composed of catalytic A and non-catalytic homo-pentameric B subunits and causes diarrheal disease in human and animals. In order to produce a nontoxic LT for vaccine... -
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- A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1
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Xiao B, Kuang Z, Zhan Y, Chen D, Gao Y, Li M, Luo S, Hao W
- KMID: 2374013
- Korean J Parasitol.
- 2016 Feb;54(1):21-29.
- doi: 10.3347/kjp.2016.54.1.21
The sodium hydrogen exchanger 1 (NHE1), which functions in maintaining the ratio of Na+ and H+ ions, is widely distributed in cell plasma membranes. It plays a prominent role in... -
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- A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice
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Park HK, Cho MK, Park MK, Kang SA, Kim YS, Kim KU, Lee MK, Ock MS, Cha HJ, Yu HS
- KMID: 1715742
- Korean J Parasitol.
- 2011 Dec;49(4):373-380.
- doi: 10.3347/kjp.2011.49.4.373
We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means... -
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- Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35
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Jung MJ, Moon YC, Cho IH, Yeh JY, Kim SE, Chang WS, Park SY, Song CS, Kim HY, Park KK, McOrist S, Choi IS, Lee JB
- KMID: 1106070
- J Vet Sci.
- 2005 Mar;6(1):21-24.
Immunocastration is a considerable alternative to a surgical castration method especially in male animal species for alleviating unwanted male behaviors and characteristics. Induction of high titer of antibody specific for... -
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- Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri
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Jeong SR, Kang SY, Lee SC, Song KJ, Im KI, Shin HJ
- KMID: 1720481
- Korean J Parasitol.
- 2004 Mar;42(1):35-40.
- doi: 10.3347/kjp.2004.42.1.35
The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed... -
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- Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application
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Lee DY, Cho YW, Kang SG, Shin SJ, Yoo HS
- KMID: 1093629
- J Vet Sci.
- 2004 Dec;5(4):337-343.
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and... -
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- Isotype and IgG Subclass Distribution of Autoantibody Response to Alpha-enolase Protein in Adult Patients with Severe Asthma
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Lee HA, Kwon B, Hur GY, Choi SJ, Nahm DH, Park HS
- KMID: 1782940
- Yonsei Med J.
- 2008 Dec;49(6):923-930.
- doi: 10.3349/ymj.2008.49.6.923
PURPOSE: A possible involvement of autoimmune mechanism in the pathogenesis of bronchial asthma has been proposed. Recently, alpha-enolase protein was identified as a major autoantigen recognized by circulating IgG autoantibodies... -
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- Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain
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Park GS, Lim SI, Hong SH, Song JY
- KMID: 1365007
- J Vet Sci.
- 2012 Mar;13(1):81-91.
- doi: 10.4142/jvs.2012.13.1.81
Classical swine fever virus (CSFV) causes a highly contagious disease among swine that has an important economic impact worldwide. CSFV strain LOM is an attenuated virus of low virulent strain... -
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