J Korean Med Sci.  1990 Mar;5(1):25-31. 10.3346/jkms.1990.5.1.25.

Antibody Response of Mouse Splenocytes Using Mixture of Supernatants of Thymocytes, Adherent and Non-adherent Splenocytes: In-vitro Immunization-II

• Kim D
• Noh GW
• Kim DH
• Kwon OH

• KMID: 1421475
• DOI: http://doi.org/10.3346/jkms.1990.5.1.25

Abstract

We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.

Keyword

In-vitro immunization; ELISA; Growth hormone

MeSH Terms

Animals
*Antibody Formation
Antigens/immunology
Cell Adhesion
Female
Growth Substances/*immunology
Immunization
Lymphocyte Activation/immunology
Mice
Mice, Inbred BALB C
Recombinant Proteins/immunology
Spleen/cytology/*immunology
T-Lymphocytes/*immunology
Antigens
Growth Substances
Recombinant Proteins