Korean J Parasitol.  2015 Jun;53(3):349-353. 10.3347/kjp.2015.53.3.349.

Anisakis pegreffii Larvae in Sea Eels (Astroconger myriaster) from the South Sea, Republic of Korea

• Cho J
• Lim H
• Jung BK
• Shin EH
• Chai JY

• KMID: 2381841
• DOI: http://doi.org/10.3347/kjp.2015.53.3.349

Abstract

Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.

Keyword

Anisakis pegreffii; sea eel; PCR-RFLP; ITS1; cox2

MeSH Terms

Animals
Anisakiasis/parasitology/*veterinary
Anisakis/classification/genetics/*isolation & purification
DNA, Helminth/genetics
DNA, Ribosomal Spacer/genetics
*Eels/growth & development
Fish Diseases/*parasitology
Larva/classification/genetics
Phylogeny
Polymorphism, Restriction Fragment Length
Republic of Korea
DNA, Helminth
DNA, Ribosomal Spacer