Korean J Nucl Med.  2003 Jun;37(3):190-198.

Radioresistance of Dendritic Cells

• Kim ES
• Kim CS
• Li MH
• Bom HS
• Min JJ
• Jeong HJ
• Kim SM
• Song HC
• Lee JJ

Abstract

PURPOSE
To evaluate radiation sensitivity of dendritic cells in comparison with lymphocytes. MATERIALS AND METHODS: T lymphocytes captured from peripheral blood were irradiated by 0 Gy, 10 Gy, 30 Gy. Apoptosis was measured by flowcytometry for staining of Annexin V 4 hours after irradiation. Immature and mature dendritic cells processed from blood hematopoietic stem cell were irradiated by 0 Gy, 10 Gy, 30 Gy, 100 Gy respectively and apoptosis was measured by flowcytometry with time difference as 4h, 24h and 48h after irradiation. Morphometric analysis by percent nucleus was measured in three cell groups, also. RESULTS: Lymphocytes showed radiation sensitivity by increasing apoptotic fraction according to radiation dose. However, both mature and immature dendritic cells showed consistent fraction of apoptosis in spite of increasing radiation dose. Percent nucleus ratio is significantly higher in lymphocytes than that of mature or immature dendritic cells. Stimulation of T-cell by dendritic cells was not changed after irradiation. CONCLUSION: Dendritic cells showed radioresistance which was associated with small size of nucleus in comparison with lymphocytes and this result would be used as a basal data of radio-labelling for the cellular trafficking studies in nuclear medicine fields.

Keyword

Radiation; Sensitivity; Hematopoietic seteam cell; Differentiatio; Dendritic cell; Lymphocyte. of arthritis

MeSH Terms

Annexin A5
Apoptosis
Dendritic Cells*
Hematopoietic Stem Cells
Lymphocytes
Nuclear Medicine
Radiation Tolerance
T-Lymphocytes
Annexin A5