Korean J Phys Anthropol.
2006 Sep;19(3):223-233.
Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier
- Affiliations
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- 1Department of Anatomy, College of Medicine, Chung-Ang University, Korea. skull@cau.ac.kr
- 2Department of Psychiatry, College of Medicine, Yonsei University, Korea.
- 3Department of Psychiatry, College of Medicine, Pochon Cha University, Korea.
- 4Department of Anatomy, College of Medicine, Kang-Won University, Korea.
- 5Division of Mathematical Sciences, College of Natural Sciences, Pukyung National University, Korea.
Abstract
- Vitamin E is the most important lipid-soluble antioxidant in humans. Although alpha-tocopherol is suggested that it has protective effect from many diseases, little is known about the prevention of occludin alteration in tight junction of blood-brain barrier (BBB) under pathologic insults producing reactive oxygen species (ROSs). In this study, the effects of alpha-tocopherol on H2O2-induced tight junction occludin were studied. Primary culture of rat brain microvessel endothelial cells was investigated with confocal microscopy, Western blot, and cell viability assay. Alpha-tocopherol had no apparent cytotoxicity up to 2.8 mM. The preincubation with alpha-tocopherol suppressed the H2O2-induced cytotoxicity in Alamar Blue assay and phase contrast microscopy. In confocal laser microscopy and Western blot, H2O2-induced loss of occludin was suppressed by preincubation with alpha-tocopherol. The present findings provide evidence that alpha-tocopherol may be beneficial for cellular protection from pathologic insults. Since alpha-tocopherol was demonstrated to have far fewer adverse effects, it would become a noteworthy nutrient or drug for the treatment of neurodegenerative diseases.
Keyword
MeSH Terms
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alpha-Tocopherol*
Animals
Blood-Brain Barrier*
Blotting, Western
Brain
Cell Survival
Endothelial Cells
Humans
Microscopy, Confocal
Microscopy, Phase-Contrast
Microvessels
Neurodegenerative Diseases
Occludin*
Rats
Reactive Oxygen Species
Tight Junctions*
Vitamin E
Vitamins
Occludin
Reactive Oxygen Species
Vitamin E
Vitamins
alpha-Tocopherol
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