J Bacteriol Virol.
2005 Sep;35(3):209-216.
A Bacterial Flagellin, Vibrio vulnificus FlaB, Induces Human Dendritic Cell Maturation
- Affiliations
-
- 1Department of Dental Pharmacology, Chonnam Dental Research Institute, School of Dentistry, Chonnam National University, Gwangju 500-757, South Korea. selee@chonnam.ac.kr
- 2Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Korea.
- 3National Research Laboratory of Molecular Microbial Pathogenesis and Department of Microbiology, Chonnam National University Medical School, Gwangju 501-746, South Korea.
- 4Department of Internal Medicine, Chonnam National University Hwasun Hospital, 160 Ilsim-ri, Hwasoon, Chonnam 519-809, South Korea.
Abstract
- The motile marine bacterium, Vibrio vulnificus has a total of six flagellins. Flagellin is a structural component of flagellar filament in various locomotive bacteria and is the ligand of Toll-like receptor 5 (TLR5). TLRs, highly expressed on various types of cells including dendritic cells (DCs), recognize invading microorganisms and finally trigger host immune responses. In this study, we prepared all of six recombinant flagellin proteins and assessed the effect of six flagellins on IL-8 activation through TLR5 recognition. Although showed different activities, five out of the six flagellins stimulated significant IL-8 activation. We also investigated the immunomodulatory roles of Vv-FlaB, the crucial building block of V. vulnificus flagellar filament, on human dendritic cells. Treatment of immature DCs with Vv-FlaB resulted in an increased expression of co-stimulatory molecules and induced strong allo-T cell proliferative activities of the DCs. These results show that the Vv-FlaB may serve an epochal immune adjuvant possessing effective immunomodulatory activities.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of the Heat Shock Protein 70 on the Adjuvanticity Induced by a Bacterial Flagellin of Vibrio ulnificus
- Tetanus toxin fragment C fused to flagellin makes a potent mucosal vaccine
- A Fusion Protein of Derp2 Allergen and Flagellin Suppresses Experimental Allergic Asthma
- Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0
- Intranasal immunization with a flagellin-adjuvanted peptide anticancer vaccine prevents tumor development by enhancing specific cytotoxic T lymphocyte response in a mouse model
