Allergy Asthma Immunol Res.  2019 Mar;11(2):254-266. 10.4168/aair.2019.11.2.254.

A Fusion Protein of Derp2 Allergen and Flagellin Suppresses Experimental Allergic Asthma

Affiliations
  • 1Clinical Vaccine R&D Center and Department of Microbiology, Chonnam National University Medical School, Gwangju, Korea. jhrhee@chonnam.ac.kr
  • 2Laboratory of In Vivo Molecular imaging, Institute for Molecular Imaging and Theranostics, Chonnam National University Medical School, Gwangju, Korea.
  • 3Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
  • 4Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Korea. selee@chonnam.ac.kr

Abstract

PURPOSE
The house dust mite (HDM) is one of the most important sources of indoor allergens and a significant cause of allergic rhinitis and allergic asthma. Our previous studies demonstrated that Vibrio vulnificus flagellin B (FlaB) plus allergen as a co-treatment mixture improved lung function and inhibited eosinophilic airway inflammation through the Toll-like receptor 5 signaling pathway in an ovalbumin (OVA)- or HDM-induced mouse asthma model. In the present study, we fused the major mite allergen Derp2 to FlaB and compared the therapeutic effects of the Derp2-FlaB fusion protein with those of a mixture of Derp2 and FlaB in a Derp2-induced mouse asthma model.
METHODS
BALB/c mice sensitized with Derp2 + HDM were treated with Derp2, a Derp2 plus FlaB (Derp2 + FlaB) mixture, or the Derp2-FlaB fusion protein 3 times at 1-week intervals. Seven days after the final treatment, the mice were challenged intranasally with Derp2, and airway responses and Derp2-specific immune responses were evaluated.
RESULTS
The Derp2-FlaB fusion protein was significantly more efficacious in reducing airway hyperresponsiveness, lung eosinophil infiltration, and Derp2-specific IgE than the Derp2 + FlaB mixture.
CONCLUSIONS
The Derp2-FlaB fusion protein showed a strong anti-asthma immunomodulatory capacity, leading to the prevention of airway inflammatory responses in a murine disease model through the inhibition of Th2 responses. These findings suggest that the Derp2-FlaB fusion protein would be a promising vaccine candidate for HDM-mediated allergic asthma therapy.

Keyword

Asthma; Derp2; FlaB flagellin; fusion protein; Toll-like receptor 5

MeSH Terms

Allergens
Animals
Asthma*
Eosinophils
Flagellin*
Immunoglobulin E
Inflammation
Lung
Mice
Mites
Ovalbumin
Pyroglyphidae
Rhinitis, Allergic
Therapeutic Uses
Toll-Like Receptor 5
Vibrio vulnificus
Allergens
Flagellin
Immunoglobulin E
Ovalbumin
Therapeutic Uses
Toll-Like Receptor 5
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