Biomol Ther.  2013 May;21(3):210-215.

Protective Effect of Fisetin (3,7,3',4'-Tetrahydroxyflavone) against gamma-Irradiation-Induced Oxidative Stress and Cell Damage

• Piao MJ
• Kim KC
• Chae S
• Keum YS
• Kim HS
• Hyun JW

Abstract

Ionizing radiation can induce cellular oxidative stress through the generation of reactive oxygen species, resulting in cell damage and cell death. The aim of this study was to determine whether the antioxidant effects of the flavonoid fisetin (3,7,3',4'-tetrahydroxyflavone) included the radioprotection of cells exposed to gamma-irradiation. Fisetin reduced the levels of intracellular reactive oxygen species generated by gamma-irradiation and thereby protected cells against gamma-irradiation-induced membrane lipid peroxidation, DNA damage, and protein carbonylation. In addition, fisetin maintained the viability of irradiated cells by partially inhibiting gamma-irradiation-induced apoptosis and restoring mitochondrial membrane potential. These effects suggest that the cellular protective effects of fisetin against gamma-irradiation are mainly due to its inhibition of reactive oxygen species generation.

Keyword

Apoptosis; Cell damage; Fisetin; gamma-irradiation; Reactive oxygen species

MeSH Terms

Antioxidants
Apoptosis
Cell Death
DNA Damage
Lipid Peroxidation
Membrane Potential, Mitochondrial
Membranes
Oxidative Stress*
Protein Carbonylation
Radiation, Ionizing
Reactive Oxygen Species
Antioxidants
Reactive Oxygen Species