Int J Oral Biol.  2011 Sep;36(3):149-154.

Protective Effects of Alpinia katsumadai Extract Against Oxidative Stress

• Lee EJ
• Kim JH

Abstract

In the present study, total methanol extracts prepared from Alpinia katsumadai showed significant protective effects against the oxidative stress induced by hydrogen peroxide, UV-C or gamma-ray irradiation. These protective effects were substantially increased by treatment with 20~100 microg/ml of the extract. The A. katsumadai total methanol preparation was further fractionated into n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these five fractions, the ethylacetate and butanol fractions of A. katsumadai showed the strongest protective effects against oxidative stress induced by UV-C and gamma-ray irradiation. These fractions also showed high DPPH radical scavenging and lipid peroxidation inhibitory activities. In addition, both fractions displayed cell proliferation activation effects, as evidenced by significant increases in colony formation. Our current data thus suggest that the mechanisms underlying the protective effects of A. katsumadai against oxidative damage may include radical scavenging, protection against cell membrane damage and stimulation of cell proliferation.

Keyword

medicinal plant extract; protection; reactive oxygen species

MeSH Terms

1-Butanol
Alpinia
Cell Membrane
Cell Proliferation
Hexanes
Hydrogen Peroxide
Lipid Peroxidation
Methanol
Methylene Chloride
Oxidative Stress
Reactive Oxygen Species
Water
1-Butanol
Hexanes
Hydrogen Peroxide
Methanol
Methylene Chloride
Reactive Oxygen Species
Water