Korean J Dermatol.
2008 Sep;46(9):1186-1193.
The Effects of Adipose Tissue-Derived Mesenchymal Stem Cells on the Formation ofEpidermis and Basement Membrane in Artificial Skin Models
- Affiliations
-
- 1Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea. khcho@snu.ac.kr
- 2Department of Dermatology, Dongguk University International Hospital, College of Medicine, Dongguk University, Goyang, Korea.
Abstract
- BACKGROUND
There is an increasing need for making a more ideal artificial skin model.
OBJECTIVE
To evaluate the effects of adipose tissue-derived mesenchymal stem cells (ATMSC) on the formation of epidermis and basement membrane in artificial skin models.
METHODS
ATMSC were isolated from lipo-aspirated fat tissues, and their phenotypes were confirmed by cell surface markers. Three kinds of artificial skin models were made using three different dermal substitutes. The dermal substitutes in the three models contained fibroblasts only, fibroblasts together with ATMSC or ATMSC only. The formation of epidermis and basement membrane was evaluated by immunohistochemical stains and transmission electron microscopy.
RESULTS
Among the three models, the model with both fibroblasts and ATMSC in the dermal substitute showed the most excellent formation of epidermis and, especially, basement membrane. In this model, the basement membrane proteins, laminin and type IV collagen, were expressed most apparently at the dermo-epidermal junction and, lamina lucida, lamina densa and anchoring fibrils were most evidently observed under transmission electron microscopy. Whereas, the model with only ATMSC did not show keratin 1 expression, suggesting that the 'skin-type' stratified squamous epithelium was not formed well.
CONCLUSION
ATMSC together with fibroblasts can be used effectively in constructing artificial skin models.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Adipose Tissue - Adequate, Accessible Regenerative Material
- Concise Review: Differentiation of Human Adult Stem Cells Into Hepatocyte-like Cells In vitro
- Adipose Tissue Derived Mesenchymal Stem Cells
- Adipose-derived stem cells: characterization and clinical application
- Comparison with human amniotic membrane- and adipose tissue-derived mesenchymal stem cells
