J Korean Soc Pediatr Endocrinol.  2011 Apr;16(1):24-30. 10.6065/jkspe.2011.16.1.24.

The Effect of IGFBP-3 on Adipokines and Gene Expression in Differentiated 3T3-L1 Adipocytes

• Kim HS

• KMID: 1450620
• DOI: http://doi.org/10.6065/jkspe.2011.16.1.24

Abstract

PURPOSE
IGFBP-3 leads to the induction of insulin resistance in 3T3-L1 adipocytes. We carried out a series of experiments to elucidate the effects of IGFBP-3 on adipokines and gene expressions.
METHODS
We treated fully-differentiated 3T3-L1 adipocytes with IGFBP-3 (0.5, 1, and 2 microg/mL) for one day and measured the mRNA levels of adiponectin, leptin, resistin, and TNF-alpha by RT-PCR, and adiponectin, leptin, resistin, and IL-6 protein levels in the culture supernatant were measured using multiplex adipokine assay ELISA Kits (Linco Research, St. Charles, Missouri). Gene expression in 3T3-L1 adipocyte cells using a microarray method was performed.
RESULTS
IGFBP-3 inhibited the expression of adiponectin, leptin, resistin, and TNF-alpha mRNA. IGFBP-3 at 0.5 and 1 micro/mL decreased adiponectin release, but IL-6 release was increased at 2 micro/mL IGFBP-3. A dose-dependent inhibition of leptin was released by IGFBP-3 at 50%. Resistin release was decreased by 40%. The effect of IGFBP-3 on the gene expression in 3T3-L1 adipocyte cells using a microarray assay related to an increase of agouti-realted proteins (Agrp) and Janus kinase 2 (JAK2), and a decrease of the ras homolog gene family (Rhoq), acyl-CoA synthetase long-chain family member 6 (Acsl6), and the interleukin-1 receptor-associated kinase 1 (Irak1).
CONCLUSION
IGFBP-3 regulates several adipokines gene expressions that are known to modulate insulin sensitivity, and this regulation may be attributable to the insulin resistance effect of IGFBP-3 on adipocytes.

Keyword

Insulin-like growth factor binding protein 3; Insulin resistance; Adipokines

MeSH Terms

Adipocytes
Adipokines
Adiponectin
Enzyme-Linked Immunosorbent Assay
Gene Expression
Humans
Insulin Resistance
Insulin-Like Growth Factor Binding Protein 3
Interleukin-1 Receptor-Associated Kinases
Interleukin-6
Janus Kinase 2
Leptin
Ligases
Proteins
Resistin
RNA, Messenger
Tumor Necrosis Factor-alpha
Adipokines
Adiponectin
Insulin-Like Growth Factor Binding Protein 3
Interleukin-1 Receptor-Associated Kinases
Interleukin-6
Janus Kinase 2
Leptin
Ligases
Proteins
RNA, Messenger
Resistin
Tumor Necrosis Factor-alpha