J Korean Soc Pediatr Endocrinol.  2011 Apr;16(1):13-19. 10.6065/jkspe.2011.16.1.13.

Analysis of Cytokines in Sera from Type 1 Diabetic Patients at Diagnosis

• Yoon IS
• Shin CH
• Yang SW

• KMID: 1450618
• DOI: http://doi.org/10.6065/jkspe.2011.16.1.13

Abstract

PURPOSE
Diverse cytokines influence the pathogenesis of type 1 diabetes mellitus (T1DM) in different ways. We studied the profile of cytokines in sera from type 1 diabetic patients at diagnosis.
METHODS
Serum levels of 11 cytokines (IL-1alpha, IL-1beta, IL-1Ra, IL-2, IL-4, IL-6, IL-10, IL-12 (p70), INF-gamma, and TNF-alpha) from 38 newly-diagnosed T1DM patents and 39 healthy controls were measured, using multiplex immunoanalytic xMAP.
RESULTS
Patients showed significantly higher levels of IL-1beta (P < 0.01), IL-10 (P < 0.01), and TNF-alpha (P = 0.019), than the healthy controls. In 12 of 35 patients, the insulin autoantibody (IAA) was positive (34%) and the level of IAA was correlated with IL-10 (r = 0.454, P = 0.006), and TNF-alpha (r = 0.368, P = 0.030).
CONCLUSION
These results suggest that IL-1beta, TNF-alpha, and IL-10 play a role in the pathogenesis of T1DM, and the level of the IAA is correlated with IL-10 and TNF-alpha.

Keyword

Diabetes mellitus, type 1; Cytokines; Interleukin-1beta; Tumor necrosis factor-alpha; Interleukin-10; Islet cell antibody

MeSH Terms

Autoantibodies
Cytokines
Diabetes Mellitus, Type 1
Humans
Insulin
Interleukin 1 Receptor Antagonist Protein
Interleukin-10
Interleukin-12
Interleukin-1beta
Interleukin-2
Interleukin-4
Interleukin-6
Tumor Necrosis Factor-alpha
Autoantibodies
Cytokines
Insulin
Interleukin 1 Receptor Antagonist Protein
Interleukin-10
Interleukin-12
Interleukin-1beta
Interleukin-2
Interleukin-4
Interleukin-6
Tumor Necrosis Factor-alpha