J Korean Neurosurg Soc.  2011 Jan;49(1):8-12. 10.3340/jkns.2011.49.1.8.

Association of Toll-Like Receptor 5 Gene Polymorphism with Susceptibility to Ossification of the Posterior Longitudinal Ligament of the Spine in Korean Population

• Chung WS
• Nam DH
• Jo DJ
• Lee JH

• KMID: 1442686
• DOI: http://doi.org/10.3340/jkns.2011.49.1.8

Abstract

OBJECTIVE
Ossification of the posterior longitudinal ligament (OPLL) has a strong genetic component. Specific gene polymorphisms may be associated with OPLL in several genes which regulate calcification in chondrocytes, change of extracellular collagen matrix and secretions of many growth factors and cytokines controlling bone morphogenesis. Toll-like receptor 5 (TLR5) may play a role in the pathogenesis of OPLL by intermediate nuclear factor-kappa B (NF-kappaB). The current study focused on coding single nucleotide polymorphisms (SNPs) of TLR5 for a case-control study investigating the relationship between TLR5 and OPLL in a Korean population.
METHODS
A total of 166 patients with OPLL and 231 controls were recruited for a case-control association study investigating the relationship between SNPs of TLR5 gene and OPLL. Four SNPs were genotyped by direct sequencing (rs5744168, rs5744169, rs2072493, and rs5744174). SNP data were analyzed using the SNPStats, SNPAnalyzer, Haploview, and Helixtree programs. Multiple logistic regression analysis with adjustment for age and gender was performed to calculate an odds ratio (OR).
RESULTS
None of SNPs were associated with OPLL in three alternative models (codominant, dominant, and recessive models; p > 0.05). A strong linkage disequilibrium block, including all 4 SNPs, was constructed using the Gabriel method. No haplotype was significantly associated with OPLL in three alternative models.
CONCLUSION
These results suggest that Toll-like receptor 5 gene may not be associated with ossification of the posterior longitudinal ligament risk in Korean population.

Keyword

OPLL; TLR5 gene; Polymorphisms; Genetic association study

MeSH Terms

Case-Control Studies
Chondrocytes
Clinical Coding
Collagen
Cytokines
Genetic Association Studies
Haplotypes
Humans
Intercellular Signaling Peptides and Proteins
Linkage Disequilibrium
Logistic Models
Longitudinal Ligaments
Morphogenesis
Odds Ratio
Ossification of Posterior Longitudinal Ligament
Polymorphism, Single Nucleotide
Spine
Toll-Like Receptor 5
Toll-Like Receptors
Collagen
Cytokines
Intercellular Signaling Peptides and Proteins
Ossification of Posterior Longitudinal Ligament
Toll-Like Receptor 5
Toll-Like Receptors