Exp Mol Med.  2006 Feb;38(1):100-106.

Naltrexone influences protein kinase C epsilon and integrin alpha7 activity in SH-SY5Y neuroblastoma cells

• Oh DY
• Jung KH
• Yang BH
• Lee JS
• Choi IG
• Chai YG

Abstract

Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKC epsilon increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKC epsilon. Integrin alpha7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKC epsilon neuronal signaling system and endothelial adhesion molecule integrin alpha7 in addition to the well-known opioid system.

Keyword

alcohols; DNA microarray; ITGA7 protein; human; naltrexone; neuroblastoma; protein kinase C

MeSH Terms

Antigens, CD/*metabolism
Cell Line, Tumor
Comparative Study
DNA, Complementary/genetics
Humans
Integrin alpha Chains/*metabolism
Naltrexone/*pharmacology
*Neuroblastoma/enzymology/metabolism/pathology
Oligonucleotide Array Sequence Analysis
Protein Kinase C-epsilon/*metabolism
Research Support, Non-U.S. Gov't
Reverse Transcriptase Polymerase Chain Reaction
Time Factors