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Establishment and evaluation of a murine alphavbeta3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus

Zhang W, Lian K, Yang F, Yang Y, Zhu Z, Zhu Z, Cao W, Mao R, Jin Y, He J, Guo J, Liu X, Zheng H

Integrin alphavbeta3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of alphavbeta3 integrin, a stable CHO-677 cell line expressing...
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Identification and Molecular Characterization of Parkin in Clonorchis sinensis

Bai X, Kim TI, Lee JY, Dai F, Hong SJ

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation...
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Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis

Hu Y, Zhang J, Yang S, Wang H, Zeng H, Zhang T, Liu J

Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against...
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Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

Theerawatanasirikul S, Suriyaphol G, Thanawongnuwech R, Sailasuta

The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5),...
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Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain

Park GS, Lim SI, Hong SH, Song JY

Classical swine fever virus (CSFV) causes a highly contagious disease among swine that has an important economic impact worldwide. CSFV strain LOM is an attenuated virus of low virulent strain...
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Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus

Sun W, Song X, Yan R, Xu L, Li X

The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5'- and 3'-ends of...
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Tissue-specific expression and subcellular localization of ALADIN, the absence of which causes human triple A syndrome

Cho AR, Yang KJ, Bae Y, Bahk YY, Kim E, Lee H, Kim JK, Park W, Rhim H, Choi SY, Imanaka T, Moon S, Yoon J, Yoon SK

Triple A syndrome is a rare genetic disorder caused by mutations in the achalasia-addisonianism-alacrima syndrome (AAAS) gene which encodes a tryptophan aspartic acid (WD) repeat-containing protein named alacrima-achalasia-adrenal insufficiency neurologic...
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Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Choi JH, Lee JH, Yu HS, Jeong HJ, Kim J, Hong YC, Kong HH, Chung DI

The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and...
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Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus

Muleke CI, Ruofeng Y, Lixin X, Yanming S, Xiangrui L

Because of the complexity of the cathepsin B-like (CBL) family, an information on the biological and biochemical characteristics of individual CBL genes is lacking. In this study, we investigated the...
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Delayed allograft rejection by the suppression of class II transactivator

Kim TW, Choi YM, Seo JN, Kim JH, Suh YH, Chung DH, Jung KC, Oh KI

  • KMID: 1094146
  • Exp Mol Med.
  • 2006 Jun;38(3):210-216.
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting...
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Role of Staphylococcal Superantigen in Atopic Dermatitis: Influence on Keratinocytes

Kim KH, Han JH, Chung JH, Cho KH, Eun HC

Staphylococcus aureus may perform an crucial function in atopic dermatitis (AD), via the secretion of superantigens, including staphylococcal enterotoxins (SE) A or B, and toxic shock syndrome toxin-1 (TSST-1). Dysregulated...
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Naltrexone influences protein kinase C epsilon and integrin alpha7 activity in SH-SY5Y neuroblastoma cells

Oh DY, Jung KH, Yang BH, Lee JS, Choi IG, Chai YG

  • KMID: 1105282
  • Exp Mol Med.
  • 2006 Feb;38(1):100-106.
Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We...
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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Ock MS, Kim BJ, Kim SM, Byun KH

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863...
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Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart

Fauchon MA, Pell TJ, Baxter GF, Yellon DM, Latchman D, Hubank MF, Mayn LV

  • KMID: 1093466
  • Exp Mol Med.
  • 2005 Aug;37(4):311-322.
Preconditioning of the myocardium rapidly induces a number of transcription factors, which are likely to be responsible for a cascade of transcriptional changes underlying the development of delayed adaptation. Identifying...
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The Effects of Intradermal Vaccination with DNA Encoding for the T-cell Receptor on the Induction of Experimental Autoimmune Encephalomyelitis in B10.PL Mice

Kwon SS, Kim N, Yoo TJ

Intradermal gene administration was found to induce a more profound immune response than direct intramusclular gene injection. We performed intradermal vaccination of B10.PL mice with DNA encoding for the V...
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Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Lee DY, Cho YW, Kang SG, Shin SJ, Yoo HS

  • KMID: 1093629
  • J Vet Sci.
  • 2004 Dec;5(4):337-343.
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and...
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Search for Genes Potentially Related to Germ Tube Formation in Candida albicans by Differential-Display Reverse Transcription Polymerase Chain Reaction

Park JY, Kim DH, Shin WS, Seo HS, Kim KH, Lee KH, Koh CM, Bong JP

Candida albicans exhibits the ability to grow in either a yeast or a mycelia form in response to different environmental factors. The mycelia form, found in infected tissues, is important...
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Sequence Analyses of Aberrant FHIT Transcripts in Gastric Cancer Cell Lines

Lee SH, Kim HS

  • KMID: 1066038
  • Korean J Gastroenterol.
  • 2003 Dec;42(6):476-483.
BACKGROUND/AIMS: The fragile histidine triad (FHIT) gene located at chromosome 3p14.2, is a candidate tumor suppressor gene often involved in various tumors. Homozygous deletions, lack or reduced expression of...
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Gene expression profiling of oxidative stress on atrial fibrillation in humans

Kim YH, Lee JH, Lim DS, Shim WJ, Ro YM, Park GH, Becker KG, Cho-Chung , Kim MK

  • KMID: 1093605
  • Exp Mol Med.
  • 2003 Oct;35(5):336-349.
Atrial Fibrillation (AF) is thought be caused by oxidative stress. Oxidative stress at the cellular level results from many factors, including exposure to alcohol, medications, cold, toxins or radiation. In...
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Overexpression of neurogenin1 induces neurite outgrowth in F11 neuroblastoma cells

Kim SY, Ghil SH, Kim SS, Myeong HH, Lee YD, Suh-Kim HY

  • KMID: 1121101
  • Exp Mol Med.
  • 2002 Dec;34(6):469-475.
Neurogenin1 (Ngn1) is a basic helix-loop-helix (bHLH) transcription factor expressed in neuronal precursors in the developing nervous system. The function of Ngn1 in neurogenesis has been shown in various...
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