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Mass spectrometry based cellular phosphoinositides profiling and phospholipid analysis: A brief review

Kim Y, Shanta SR, Zhou LH, Kim KP

Phospholipids are key components of cellular membrane and signaling. Among cellular phospholipids, phosphoinositides, phosphorylated derivatives of phosphatidylinositol are important as a participant in essential metabolic processes in animals. However, due...
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Application of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Imaging Mass Spectrometry (MALDI-TOF IMS) for Premalignant Gastrointestinal Lesions

Ko KH, Kwon CI, Park SH, Han NY, Lee HK, Kim EH, Hahm KB

  • KMID: 2165420
  • Clin Endosc.
  • 2013 Nov;46(6):611-619.
Imaging mass spectrometry (IMS) is currently receiving large attention from the mass spectrometric community, although its use is not yet well known in the clinic. As matrix-assisted laser desorption/ionization time-of-flight...
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Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Lee Y, Kim N, Kim H, Joo H, Kim Y, Jeong D, Cuong DV, Kim E, Hur DY, Park YS, Hong YG, Lee S, Chung J, Seog D, Han J

  • KMID: 1611348
  • Korean J Physiol Pharmacol.
  • 2004 Aug;8(4):207-211.
The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR)...
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Two Cases of Clostridium tertium Infection and Successful Identification of the Organism by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry Analysis

Joichi Y, Kayama S, Hayashi I, Onodera M, Furushimo , Koba Y, Yokozaki M, Ohge H, Sugai M

No abstract available.
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Comparison of the Bruker Biotyper and VITEK MS Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Systems Using a Formic Acid Extraction Method to Identify Common and Uncommon Yeast Isolates

Lee HS, Shin JH, Choi MJ, Won EJ, Kee SJ, Kim SH, Shin MG, Suh SP

BACKGROUND: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with...
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Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria

Lee W, Kim M, Yong D, Jeong SH, Lee K, Chong Y

BACKGROUND: By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight...
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Clinical efficacy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in patients with multidrug-resistant bacteremia: a single-center study in Korea

Mok J, Jo EJ, Eom JS, Kim MH, Kim KU, Park HK, Lee MK, Lee K

BACKGROUND/AIMS: Matrix-assisted laser desorption/ionization time-of-f light mass spectrometry (MALDI-TOF MS) is a new diagnostic tool for microorganism identification. The clinical usefulness of this approach has not been widely examined in...
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Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based VITEK MS System for the Identification of Acinetobacter Species from Blood Cultures: Comparison with VITEK 2 and MicroScan Systems

Lee SY, Shin JH, Kim SH, Shin MG, Suh SP, Ryang DW

BACKGROUND: Acinetobacter species are the leading cause of bloodstream infection (BSI), but their correct identification is challenging. We evaluated the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based...
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Imaging Mass Spectrometry in Papillary Thyroid Carcinoma for the Identification and Validation of Biomarker Proteins

Min KW, Bang JY, Kim KP, Kim WS, Lee SH, Shanta SR, Lee JH, Hong JH, Lim SD, Yoo YB, Na CH

Direct tissue imaging mass spectrometry (IMS) by matrix-assisted laser desorption ionization and time-of-flight (MALDI-TOF) mass spectrometry has become increasingly important in biology and medicine, because this technology can detect the...
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Matrix-assisted laser desorption ionization-time of flight mass spectrometry based identification of Edwardsiella ictaluri isolated from Vietnamese striped catfish (Pangasius hypothalamus)

Nhu TQ, Park SB, Kim SW, Lee JS, Im SP, Lazarte JM, Seo JP, Lee WJ, Kim JS, Jung TS

Edwardsiella (E.) ictaluri is a major bacterial pathogen that affects commercially farmed striped catfish (Pangasius hypothalamus) in Vietnam. In a previous study, 19 strains of E. ictaluri collected from striped...
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Reference Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

Park MR, Lee EG, Kim YH, Jung TS, Shin YS, Shin GW, Cha HG, Kim GS

  • KMID: 1089404
  • J Vet Sci.
  • 2003 Aug;4(2):143-149.
Protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis (2-DE)...
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Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System

Jo SJ, Park KG, Han K, Park DJ, Park YJ

BACKGROUND: We evaluated the reliability and accuracy of the combined use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial identification and Vitek 2 antimicrobial susceptibility testing (AST) for...
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Candidates for Tumor Markers of Cervical Cancer Discovered by Proteomic Analysis

Song JY, Bae HS, Koo DH, Lee JK, Jung HH, Lee KW, Lee NW

Cervical cancer is the second most common gynecological cancer among Korean women. While nationwide screening program has developed, the pathogenesis of cervical cancer is unknown. The aim of this study...
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Identification of Acinetobacter Species Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Jeong S, Hong JS, Kim JO, Kim KH, Lee W, Bae IK, Lee K, Jeong SH

BACKGROUND: Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted...
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Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs

Gandolfi-Decristophoris P, Regula G, Petrini O, Zinsstag J, Schelling

We investigated the distribution of commensal staphylococcal species and determined the prevalence of multi-drug resistance in healthy cats and dogs. Risk factors associated with the carriage of multi-drug resistant strains...
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Utility of Conventional Culture and MALDI-TOF MS for Identification of Microbial Communities in Bronchoalveolar Lavage Fluid in Comparison with the GS Junior Next Generation Sequencing System

Sung JY, Hwang Y, Shin MH, Park MS, Lee SH, Yong D, Lee K

BACKGROUND: Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical...
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Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae)

Kim YH, slam MS, You MJ

Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with...
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Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy

Lee CS, Kang KR, Lee JY, Park CS, Hahn KH, Sohn JW, Kim BJ

Electroconvulsive therapy (ECT) is one of the most effective treatments used in psychiatry to date. The mechanisms of ECT action, however, are the least understood and still unclear. As a...
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The First Case of Eggerthella lenta Bacteremia in Korea

Lee HJ, Hong SK, Choi WS, Kim EC

No abstract available.
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A Potential Role of Crystallin in the Vitreous Bodies of Rats after Ischemia-reperfusion Injury

Hong SM, Yang YS

PURPOSE: Ischemia-reperfusion injury (I/R injury) is known not only to induce hypoxic and oxidative stress, but also to cause retinal degeneration in rats. Crystallins, known to inhibit the formation of...
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