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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

Choi IS, Yoo HS

  • KMID: 1095123
  • J Vet Sci.
  • 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2...
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Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues

Lee B, Kang HY, Lee DO, Ahn C, Jeung EB

Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful...
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Generation, characterization, and application in serodiagnosis of recombinant swine vesicular disease virus-like particles

Xu W, Goolia M, Salo T, Zhang Z, Yang M

Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those...
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Immunohistochemical study of osteopontin in boar testis

Kim S, Shin T

The expression of osteopontin (OPN) in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets, the...
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Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs

Alam MR, Ji JR, Kim MS, Kim NS

The levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in synovial fluid (SF) and serum in cases of canine osteoarthritis (OA)...
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Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development

Kim H, Moon C, Ahn M, Lee Y, Kim H, Kim S, Ha T, Jee Y, Shin T

  • KMID: 1114849
  • J Vet Sci.
  • 2005 Jun;6(2):97-101.
The expression of nitric oxide synthase (NOS) isoforms in the ovaries of pigs was examined to study the involvement of nitric oxide, a product of NOS activity, in the function...
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Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

Shin SJ, Bae JL, Cho YW, Yang MS, Kim DH, Jang YS, Yoo HS

  • KMID: 1089601
  • J Vet Sci.
  • 2003 Dec;4(3):225-228.
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by...
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Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT² Profiler PCR Array and immunochemistry assays

Yu F, Rasotto R, Zhang H, Pei S, Zhou B, Yang X, Jin Y, Zhang D, Lin D

The Wnt signaling pathway and its key component β-catenin have critical roles in the development of diseases such as tumors in mammals. However, little has been reported about involvement of...
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Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats

Kim H, Moon C, Kim J, Ahn M, Hyun JW, Park JW, Kim SH, Kim S, Shin T

Phosphorylation of caveolin-1 occurs during cell activation by various stimuli. In this study, the involvement of caveolin-1 in an irradiation injured spinal cord was examined by analyzing the phosphorylation of...
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Transcriptional and translational expression of calbindin-D9k in the duodenum, kidney and uterus of a female canine model

Sim JY, Jung EM, Yoo YM, Choi KC, Jeung EB

Calbindin-D9k (CaBP-9k) is a cytosolic calcium-binding protein expressed in tissues in the intestine, uterus, placenta, kidney, pituitary gland and bone. Its exact function is unknown, but it is considered to...
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Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors

Lee CH, Kim WH, Lim JH, Kang MS, Kim DY, Kweon OK

  • KMID: 1094689
  • J Vet Sci.
  • 2004 Mar;5(1):63-69.
We concentrated ourselves to evaluate the prognostic significance of the p53 gene mutations, its protein expression and MIB-1 index as a proliferative marker in canine mammary tumors. In the present...
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Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury

Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK

Heme oxygenase-1 (HO-1) is a stress-responsive enzyme that modulates the immune response and oxidative stress associated with spinal cord injury (SCI). This study aimed to investigate neuronal regeneration via transplantation...
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Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Wu LM, Guo R, Hui L, Ye YG, Xiang JM, Wan CY, Zou M, Ma R, Sun XZ, Yang SJ, Guo DZ

Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the...
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Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice

Lim JJ, Kim DH, Lee JJ, Kim DG, Min W, Lee HJ, Rhee MH, Kim S

The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study,...
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Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Lee DY, Cho YW, Kang SG, Shin SJ, Yoo HS

  • KMID: 1093629
  • J Vet Sci.
  • 2004 Dec;5(4):337-343.
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and...
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Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Sagong M, Park CK, Kim SH, Moon SU, Cho SC, Lee C

Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted...
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Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model

Oh Y, Lee J, Kim HC, Hahn TW, Yoon BI, Han JH, Kwon YS, Park JJ, Koo DB, Rhee KJ, Jung BD

Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although...
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Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro

Jung TS, Thompson K, Volpatti D, Galeotti M, Adams A

The antigenicity of Photobacterium damselae (Ph. d.)subsp. piscicida, cultured in four different growth media[tryptone soya broth (TSB), glucose-rich medium (GRM),iron-depleted TSB (TSB+IR-), and iron-depleted GRM(GRM+IR-)] was compared by enzyme-linked immuno-sorbent...
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Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

Li H, Yang J, Bao D, Hou J, Zhi Y, Yang Y, Ji P, Zhou E, Qiao S, Zhang G

A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies...
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Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors

Blanc AM, Berois MB, Tome LM, Epstein AL, Arbiza JR

Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens....
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