Exp Mol Med.  2000 Dec;32(4):210-215.

Varying expression levels of colony stimulating factor receptors in disease states and different leukocytes

• Lee KY
• Suh BG
• Kim JW
• Lee WB
• Kim SY
• Kim YY
• Lee JH
• Lim JH
• Lim MS
• Kang CS
• Han KJ

Abstract

Administration of G-CSF may not always respond in rise of neutrophil counts in different patient population. In order to understand a possible inter-relationship between the G-CSF and GM-CSF induced leukocyte responses and expression levels of receptors for G-CSF (G-CSFr) and GM-CSF (GM-CSFr), the levels of each receptor and CSF were measured in patients with basophilia (8), eosinophilia (14) and bacterial infection showing neutrophilia (12) in comparison with normal healthy adults (12) and children (14). G-CSFr was expressed in neutrophils in the largest amount followed by monocytes, but GM-CSFr was expressed more in monocytes than neutrophils. Lymphocytes and basophils did not express G-CSFr or GM-CSFr. The amount of GM-CSFr in neutrophils was present less in patients with infection than normal control (P = 0.031). The neutrophils expressed more G-CSFr than GM-CSFr. The quantity of G-CSFr in eosinophil showed marked interval change, higher in acute stage. The plasma concentrations of G-CSF in patients with infection were much higher than normal adults or children (117.95 +/- 181.16 pg/ml, P < 0.05). Binding assay with excess amount of CSFs could discriminate the patient who did not show any response to G-CSF or GM-CSF administration. After incubation with excess CSFs, more receptors were blocked in children than in adults (G-CSF P = 0.024, GM-CSF P = 0.006). These results indicate that the amount of CSFr in leukocyte varies in different types of leukocyte, and changes according to the patients' condition even in the same type of leukocyte, and the CSFrs of children bind to CSFs more than those of adults.

Keyword

Granulocyte-colony stimulating factor; granulocyte macrophage-colony stimulating factor; G-CSF receptor; GM-CSF receptor; leukocyte

MeSH Terms

Adult
*Bacterial Infections
Basophils/chemistry
Breast Neoplasms
Child
Colony-Stimulating Factors/*blood
Eosinophilia
Human
Leukemia, Myeloid, Chronic
*Leukocyte Disorders
Monocytes/chemistry
*Neoplasms
Neutrophils/chemistry
Receptors, Colony-Stimulating Factor/*analysis
Receptors, Granulocyte Colony-Stimulating Factor/analysis
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/analysis