J Korean Pediatr Soc.  2003 Apr;46(4):376-381.

Plasma G-CSF and GM-CSF Concentration and Amount of Their Receptors on the Granulocyte in Kawasaki Disease

• Yoo YK
• Lee G
• Kim HH
• Kim SY
• Kim YJ
• Lee W

Abstract

PURPOSE
This study aimed to demonstrate the possible pathogenesis of granulopoiesis in patients of Kawasaki disease(KD) using quantitative analysis of G-CSF, GM-CSF and their CSFr.
METHODS
The plasma levels of G-CSF, GM-CSF, G-CSFr and GM-CSFr were studied in 14 patients in the acute phase of KD; 13 children with normal peripheral white blood cell counts were used as the normal control group. The plasma concentration of G-CSF, GM-CSF were analyzed by ELISA. The G-CSFr and GM-CSFr on the peripheral granulocytes were analyzed by a quantitative flow cytometric assay and QuantiBRITE, and the quantitative changes of receptors which did not combine with G-CSF and GM-CSF were measured.
RESULTS
The total number of leukocytes in KD was similar to normal control group, but the leukocytes increased according to the number of neutrophils. The plasma concentration of G-CSF were decreased similar to normal control group(P=0.133), but that of GM-CSF decreased more than the normal control group(P=0.227). The quantity of G-CSFr, GM-CSFr were revealed to be no less than the normal control(P=0.721, P=0.912). After incubation with excessive G-CSF, the expressed G-CSFr on the neutrophils were decreased in both groups(P=0.554). The quantities of expressions of GM- CSFr on the neutrophil after incubation with the excessive GM-CSF were always increased in both groups(P=0.255). The amount of GM-CSFr of neutrophils are in proportion to total white blood cells (r=0.788, P=0.035), but it wasn't in the case of KD(P=0.644).
CONCLUSION
The leukocytosis in KD that mediated by increasing neutrophil was not correlated with the plasma concentrations of G-CSF and GM-CSF, and the amount of expression of G-CSFr and GM-CSFr on granulocyte. It is possible that the reduction of concentration of GM-CSF results by increasing the active GM-CSFr.

Keyword

Granulocyte-colony stimulating factor(G-CSF); Granulocyte macrophage-colony stimulating factor(GM-CSF); G-CSF receptor; GM-CSF receptor; Kawasaki disease

MeSH Terms

Child
Enzyme-Linked Immunosorbent Assay
Granulocyte Colony-Stimulating Factor*
Granulocyte-Macrophage Colony-Stimulating Factor*
Granulocytes*
Humans
Leukocyte Count
Leukocytes
Leukocytosis
Mucocutaneous Lymph Node Syndrome*
Neutrophils
Plasma*
Receptors, Granulocyte Colony-Stimulating Factor
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Granulocyte Colony-Stimulating Factor
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor