J Korean Pediatr Soc.  2003 Mar;46(3):271-276.

Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis

• Choi WS
• Ryu KH
• Kim YJ
• Kim SY
• Kim HH
• Lee W

Abstract

PURPOSE
Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr.
METHODS
The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay.
RESULTS
There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference.
CONCLUSION
Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis.

Keyword

Granulocyte-colony stimulating factor(G-CSF); Granulocyte macrophage-colony stimulating factor(GM-CSF); G-CSF receptor; GM-CSF receptor; Leukocytosis

MeSH Terms

Antibodies, Monoclonal
Child*
Communicable Diseases
Cytokines
Granulocyte Colony-Stimulating Factor*
Granulocyte-Macrophage Colony-Stimulating Factor*
Granulocytes*
Humans
Leukocytes
Leukocytosis*
Neutrophils
Plasma*
Receptors, Cell Surface
Receptors, Granulocyte Colony-Stimulating Factor
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Antibodies, Monoclonal
Cytokines
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Cell Surface
Receptors, Granulocyte Colony-Stimulating Factor
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor