J Korean Med Sci.  1999 Oct;14(5):480-486. 10.3346/jkms.1999.14.5.480.

Effects of interleukin-10 on chemokine KC gene expression by mouse peritoneal macrophages in response to Candida albicans

• Kim HS
• Shin DH
• Kim SK

• KMID: 646014
• DOI: http://doi.org/10.3346/jkms.1999.14.5.480

Abstract

Chemokine KC has been considered to be a murine homologue of human GRO/MGSA and was identified as chemoattractant for monocytes and neutrophils. This study examined the expression of KC mRNA in thioglycollate-elicited mouse peritoneal macrophages that were stimulated in vitro with Candida albicans (CA). Also examined were the inhibitory effects of IL-10 on the CA-induced expression of KC gene by Northern blot analysis. CA was found to induce chemokine gene expression in a gene-specific manner, CXC chemokine IP-10 mRNA expression was not detected in CA-stimulated macrophages. Maximum KC mRNA expression was observed approximately 2 hr after adding CA. The inhibitory action of IL-10 to CA-induced KC mRNA expression on mouse peritoneal macrophages was independent on concentration and stimulation time of IL-10 and was observed approximately one hour after adding IL-10 and CA simultaneously. IL-10 produced a decrease in the stability of KC mRNA, and CA-stimulated macrophages with cycloheximide blocked the suppressive effect of IL-10. These results suggest that CA also induces chemokine KC from macrophages, and IL-10 acts to destabilize CA-induced KC mRNA and de novo synthesis of an intermediate protein is a part of the IL-10 suppressive mechanism.

Keyword

Chemokines; Candida albicans; Gene expression

MeSH Terms

Animal
Blotting, Northern
Candida albicans/metabolism*
Cells, Cultured
Chemotactic Factors/genetics*
Dactinomycin/pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation/drug effects*
Growth Substances/genetics*
Interleukin-10/pharmacology*
Interleukin-10/metabolism
Macrophages/physiology*
Mice
Mice, Inbred BALB C
Nucleic Acid Synthesis Inhibitors/pharmacology
RNA, Messenger/metabolism
RNA, Messenger/drug effects