Yeungnam Univ J Med.  1998 Jun;15(1):47-54. 10.12701/yujm.1998.15.1.47.

The effect of interleukin-10 on KC gene expression in mouse peritoneal macrophages

• Kim HS

• KMID: 2154411
• DOI: http://doi.org/10.12701/yujm.1998.15.1.47

Abstract

Interleukin-10(IL-10) inhibits production of a wide range of cytokines in various cell types and transcriptionally inhibits lipopolysaccharide(LPS)-induced expression of proinflammatory mediators. Cytokine expression by macrophages is an important aspect to ochestrate inflammatory responses. As an approach to identify mechanistic targets of IL-10, it was examined the time course for expression of KC(murine homologue of Gro) gene in murine peritoneal macrophages stimulated with LPS with or without IL-10. The effect of IL-10 on LPS induced KC mRNA expression was delayed and only seen after 1 hour treatment. Pretreatment with IL-10 did not eliminate the delayed inhibitory response nor increase the magnitude of suppression. These effects did not depend upon time of IL-10 treatment but the time of LPS treatment. LPS-induced KC mRNA expression by inhibitoy action of IL-10 was not controlled at the level of transcription. The result indicates that IL-10 acts late in the process of KC gene expression and that the prominant site of action may be mRNA stability or translation.

Keyword

KC; interleukin-10(IL-10)

MeSH Terms

Animals
Cytokines
Gene Expression*
Interleukin-10*
Macrophages
Macrophages, Peritoneal*
Mice*
RNA Stability
RNA, Messenger
Cytokines
Interleukin-10
RNA, Messenger