J Nutr Health.  2019 Feb;52(1):26-35. 10.4163/jnh.2019.52.1.26.

Antioxidant activity of ethanol extract of Lycium barbarum's leaf with removal of chlorophyll

Affiliations
  • 1Department of Food and Nutrition, Chungam National University, Daejeon 34134, Korea. sunly@cnu.ac.kr
  • 2Convergence Research Center for Natural Products, Chungnam National University, Daejeon 34134, Korea.

Abstract

PURPOSE
The aim of this study was to estimate the antioxidant activities of 50%, 70%, and 100% ethanol extracts of Lycium barbarum leaf and chlorophyll removal extract.
METHODS
The antioxidant activities were estimated by measuring total polyphenol content and by assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2"²-azino-bis (3-ethylbenzothiazoline-6-sulfate) (ABTS) radical scavenging activities and ferric reducing antioxidant power (FRAP). In addition, reactive oxygen species (ROS) production, DNA fragmentation, and antioxidant enzyme (superoxide dismutase and catalase) activities of the extracts were measured in hydrogen peroxide (H2O2)-stressed HepG2 cells.
RESULTS
The total polyphenol content, DPPH and ABTS radical scavenging activities, and FRAP value of the extracts increased in an ethanol concentration-dependent manner. The antioxidant activities of the chlorophyll-removal extracts were much higher than those of the chlorophyll-containing extracts. Cytotoxicity was not observed in HepG2 cells with extracts up to 1,000 µg/mL. All extracts inhibited ROS production in a concentration-dependent manner from 31.3 µg/mL and inhibited DNA damage at 250 µg/mL. The SOD and catalase activities of cell lines treated with the extracts and H2O2 were similar to those of normal cells, indicating a strong protective effect.
CONCLUSION
Lycium barbarum leaf extracts had high antioxidant activities and protected H2O2-stressed HepG2 cells. Since the chlorophyll-removal extract exhibited higher antioxidant activities than the chlorophyll-containing ones and the cytoprotective effect was similar, chlorophyll removal extract of Lycium barbarum leaf could be developed as ingredients of functional food and cosmetics.

Keyword

antioxidant; Lycium barbarum; HepG2 cell; chlorophyll removal

MeSH Terms

Catalase
Cell Line
Chlorophyll*
DNA Damage
DNA Fragmentation
Ethanol*
Functional Food
Hep G2 Cells
Hydrogen Peroxide
Lycium*
Reactive Oxygen Species
Catalase
Chlorophyll
Ethanol
Hydrogen Peroxide
Reactive Oxygen Species

Figure

  • Fig. 1 Effect of the Lycium barbarum leave's ethanol extracts (LL50, LL70, LL100, LL100 Ch-) on HepG2 cell viability. LL 50, 70 and 100: Lycium barbarum's leaf extracted with 50, 70 and 100% ethanol, respectively; Group LL100 Ch-: chlorophyll removal ethanol extract. Values are mean ± standard deviation of three replicate determinations (n = 3). Different letters above the bars indicate statistically significant differences (p < 0.05).

  • Fig. 2 Effects of (A) 50% ethanol extracs, (B) 70% ethanol extract, (C) 100% ethanol extract of Lycium barbarum's leaf and (D) chlorophyll removal extract with 100% ethanol from Lycium barbarum's leaf on the production of intracellular ROS level in H2O2-treated HepG2 cells. Values are mean ± SD of three replicate determinations (n = 3). Different superscripts (a–c) in a column indicate significant differences at p < 0.05 by Duncan's multiple range test (*: p < 0.05, compared to treated only H2O2).


Cited by  1 articles

Anti-inflammatory effects of fruit and leaf extracts of Lycium barbarum in lipopolysaccharide-stimulated RAW264.7 cells and animal model
Su-Mi Bae, Ji-Eun Kim, Eun-Young Bae, Kyung-Ah Kim, Sun Yung Ly
J Nutr Health. 2019;52(2):129-138.    doi: 10.4163/jnh.2019.52.2.129.


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