J Nutr Health.  2019 Dec;52(6):529-539. 10.4163/jnh.2019.52.6.529.

Antioxidant and antiobesity activities of oral treatment with ethanol extract from sprout of evening primrose (Oenothera laciniata) in high fat diet-induced obese mice

• Kwak CS
• Kim MJ
• Kim SG
• Park S
• Kim IG
• Kang HS

• KMID: 2466667
• DOI: http://doi.org/10.4163/jnh.2019.52.6.529

Abstract

PURPOSE
Sprouts of evening primrose (Oenothera laciniata, OL) were reported to have high contents of flavonoids and potent antioxidant activity. This study examined the antioxidant and antiobesity activities of OL sprouts to determine if they could be a natural health-beneficial resource preventing obesity and oxidative stress.
METHODS
OL sprouts were extracted with 50% ethanol, evaporated, and lyophilized (OLE). The in vitro antioxidant activity of OLE was examined using four different tests. The antiobesity activity and in vivo antioxidant activity from OLE consumption were examined using high fat diet-induced obese (DIO) C57BL/6 mice.
RESULTS
The ICâ‚…â‚€ for the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activities of OLE were 26.2 µg/mL and 327.6 µg/mL, respectively. OLE exhibited the ferric reducing antioxidant power (FRAP) activity of 56.7 µg ascorbic acid eq./mL at 100 µg/mL, and an increased glutathione level by 65.1% at 200 µg/mL compared to the control in the hUC-MSC stem cells. In an animal study, oral treatment with 50 mg or 100 mg of OLE/kg body weight for 14 weeks reduced the body weight gain, visceral fat content, fat cell size, blood leptin, and triglyceride levels, as well as the atherogenic index compared to the high fat diet control group (HFC) (p < 0.05). The blood malondialdehyde (MDA) level and the catalase and SOD-1 activities in adipose tissue were reduced significantly by the OLE treatment compared to HFC as well (p < 0.05). In epididymal adipose tissue, the OLE treatment reduced the mRNA expression of leptin, PPAR-γ and FAS significantly (p < 0.05) compared to HFC while it increased adiponectin expression (p < 0.05).
CONCLUSION
OLE consumption has potent antioxidant and antiobesity activities via the suppression of oxidative stress and lipogenesis in DIO mice. Therefore, OLE could be a good candidate as a natural resource to develop functional food products that prevent obesity and oxidative stress.

Keyword

Oenothera laciniata; antioxidant; antiobesity; diet-induced obese model; adipokines

MeSH Terms

Adipocytes
Adipokines
Adiponectin
Adipose Tissue
Animals
Ascorbic Acid
Body Weight
Catalase
Diet, High-Fat
Ethanol*
Flavonoids
Functional Food
Glutathione
In Vitro Techniques
Intra-Abdominal Fat
Leptin
Lipogenesis
Malondialdehyde
Mice
Mice, Obese*
Natural Resources
Obesity
Oenothera biennis*
Oxidative Stress
RNA, Messenger
Stem Cells
Superoxide Dismutase
Triglycerides
Adipokines
Adiponectin
Ascorbic Acid
Catalase
Ethanol
Flavonoids
Glutathione
Leptin
Malondialdehyde
RNA, Messenger
Superoxide Dismutase

Figure

• 

  Fig. 1 Effect of OLE treatment on the GSH level in hUC-MSC cells. (A) GSH level according to the different passages of the cells. (B) Effect of OLE treatment on the GSH level in passage 15 cells. Each bar represents the mean ± SD. ***p < 0.001 compared with passage 4 (p4) or control.

• 

  Fig. 2 Effect of OLE oral treatment on the body weight gain of mice fed high-fat diet. Data are represented as the mean ± SD (n = 8). Means that do not share the same superscript are significantly different by ANOVA/Duncan's multiple test at p < 0.05.

• 

  Fig. 3 Effect of OLE oral treatment on the visceral adipose tissue accumulation and morphology in mice. (A) Visceral adipose tissue weight. (B) Representative H&E staining of epididymal adipose tissue and (C) size of adipocytes. Each bar represents the mean ± SD. Bars that do not share the same superscript are significantly different by ANOVA/Duncan's multiple test at p < 0.05.

• 

  Fig. 4 Effect of OLE oral treatment on the antioxidant enzyme activities in epididymal adipose tissue of mice. Each bar represents the mean ± SD. Bars that do not share the same superscript are significantly different by ANOVA/Duncan's multiple test at p < 0.05.

• 

  Fig. 5 Effect of OLE oral treatment on the mRNA of adipokines and lipid-metabolism related genes in epidydimal fat tissue of mice. Each bar represents the mean ± SD. Bars that do not share the same superscript are significantly different by ANOVA/Duncan's multiple test at p < 0.05.

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