Biomol Ther.  2019 Jul;27(4):404-413. 10.4062/biomolther.2018.195.

Udenafil Induces the Hair Growth Effect of Adipose-Derived Stem Cells

Affiliations
  • 1College of Pharmacy, Yonsei University, Incheon 21983, Republic of Korea. brian99@empal.com, nh147837@gmail.com
  • 2STEMORE Co. Ltd., Incheon 21984, Republic of Korea.

Abstract

Udenafil, which is a PDE5 inhibitor, is used to treat erectile dysfunction. However, it is unclear whether udenafil induces hair growth via the stimulation of adipose-derived stem cells (ASCs). In this study, we investigated whether udenafil stimulates ASCs and whether increased growth factor secretion from ASCs to facilitate hair growth. We found that subcutaneous injection of udenafil-treated ASCs accelerated telogen-to-anagen transition in vivo. We also observed that udenafil induced proliferation, migration and tube formation of ASCs. It also increased the secretion of growth factors from ASCs, such as interleukin-4 (IL-4) and IL12B, and the phosphorylation of ERK1/2 and NFκB. Furthermore, concomitant upregulation of IL-4 and IL12B mRNA levels was attenuated by ERK inhibitor or NFκB knockdown. Application of IL-4 or IL12B enhanced anagen induction in mice and increased hair follicle length in organ culture. The results indicated that udenafil stimulates ASC motility and increases paracrine growth factor, including cytokine signaling. Udenafil-stimulated secretion of cytokine from ASCs may promote hair growth via the ERK and NFκB pathways. Therefore, udenafil can be used as an ASC-preconditioning agent for hair growth.

Keyword

Udenafil; Adipose-derived stem cells; IL-4; IL12B; Hair growth

MeSH Terms

Animals
Erectile Dysfunction
Hair Follicle
Hair*
Injections, Subcutaneous
Intercellular Signaling Peptides and Proteins
Interleukin-4
Male
Mice
Organ Culture Techniques
Phosphorylation
RNA, Messenger
Stem Cells*
Up-Regulation
Intercellular Signaling Peptides and Proteins
Interleukin-4
RNA, Messenger
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