Ann Dermatol.  2016 Oct;28(5):662-665. 10.5021/ad.2016.28.5.662.

Poor Capability of 3D-Cultured Adipose-Derived Stem Cells to Induce Hair Follicles in Contrast to 3D-Cultured Dermal Papilla Cells

• Seo CH
• Kwack MH
• Lee SH
• Kim MK
• Kim JC
• Sung YK

• KMID: 2382902
• DOI: http://doi.org/10.5021/ad.2016.28.5.662

Abstract

No abstract available.

MeSH Terms

Hair Follicle*
Hair*
Stem Cells*

Figure

• 

  Fig. 1 Hair reconstitution assay and trichogene expression using human adipose-derived stem cells (ADSCs) and dermal papilla (DP) cells. (A) Hair induction was observed in a positive control experiment in which freshly isolated mouse dermal cells (106 cells) and epidermal cells (106 cells) were co-transplanted subcutaneously into the skin on the backs of nude mice. (B) No hair induction was observed when human ADSCs alone (106 cells) were implanted. Two-dimensional (2D)-cultured ADSCs (C) or ADSC spheres (100 in total) grown using hydrocell plates (D) were implanted together with fresh mouse epidermal cells (106 cells). As a control experiment, 2D-cultured DP cells (E) or DP spheres (100 in total) grown using hydrocell plates (F) were implanted together with fresh mouse epidermal cells (106 cells). (G) The mRNA expression of versican and Sox2 in DP spheres was compared to ADSC spheres by real-time PCR analysis. Data are means±standard deviation of triplicates per experiment from two independent experiments. The sequences of primers used in this study are as follows: Versican, Qiagen predesigned primer QT00064064; Sox2, 5'-TTTAGGACAGTTGCAAACGTGAA-3' and 5'-TCAACCTGCATGGCCATTTT-3'; GAPDH, 5'-TGGAAATCCCATCACCATCTTC-3' and 5'-CGCCCCACTTGATTTTGG-3'. (H) Immunofluorescence staining of versican (green) and the corresponding 4', 6-diamidino-2-phenylindole (DAPI) nuclear staining (blue) in DP spheres compared to ADSC spheres. Briefly, the sections were incubated with a mouse monoclonal antibody to versican (1:1,000 dilution; Seikagaku Corporation, Tokyo, Japan), washed with phosphate buffered saline, and incubated with Alexa Flour 488 labeled donkey anti-mouse secondary antibody (1:1,000 dilution; Molecular Probes, Eugene, OR, USA). The slides were then counterstained with DAPI.

Reference

1. Driskell RR, Clavel C, Rendl M, Watt FM. Hair follicle dermal papilla cells at a glance. J Cell Sci. 2011; 124:1179–1182.

2. Ohyama M, Zheng Y, Paus R, Stenn KS. The mesenchymal component of hair follicle neogenesis: background, methods and molecular characterization. Exp Dermatol. 2010; 19:89–99.

3. Ohyama M, Veraitch O. Strategies to enhance epithelialmesenchymal interactions for human hair follicle bioengineering. J Dermatol Sci. 2013; 70:78–87.

4. Kang BM, Kwack MH, Kim MK, Kim JC, Sung YK. Sphere formation increases the ability of cultured human dermal papilla cells to induce hair follicles from mouse epidermal cells in a reconstitution assay. J Invest Dermatol. 2012; 132:237–239.

5. Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, et al. Multipotential differentiation of adipose tissuederived stem cells. Keio J Med. 2005; 54:132–141.

6. Driskell RR, Jahoda CA, Chuong CM, Watt FM, Horsley V. Defining dermal adipose tissue. Exp Dermatol. 2014; 23:629–631.

7. Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, et al. Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature. 2013; 504:277–281.

8. Kwack MH, Sung YK, Chung EJ, Im SU, Ahn JS, Kim MK, et al. Dihydrotestosterone-inducible dickkopf 1 from balding dermal papilla cells causes apoptosis in follicular keratinocytes. J Invest Dermatol. 2008; 128:262–269.

9. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13:4279–4295.

10. Park KC, Choi HR, Na JI, Cho HJ, Nam KM, Choi JW, et al. Effects of murine dermal cells on the regulation of hair growth is dependent on the cell number and post-natal age of newborn mice. Ann Dermatol. 2012; 24:94–98.