J Korean Dent Sci.  2018 Dec;11(2):43-56. 10.5856/JKDS.2018.11.2.43.

Bone Regeneration Using Block-type Deproteinized Porcine Bone Mineral with Collagen Membrane Using 3,4-Dihydroxyphenylalanine as Bone Adhesive

• Kang JH
• Pae HC
• Cha JK
• Lee JS
• Paik JW
• Jung UW
• Choi SH

• KMID: 2437341
• DOI: http://doi.org/10.5856/JKDS.2018.11.2.43

Abstract

PURPOSE
The purpose of this study was to assess the adhesiveness and cytotoxicity of 3, 4-dihydroxyphenylalanine (DOPA), and to evaluate the role of collagen membrane with DOPA in the guided bone regeneration.
MATERIALS AND METHODS
Peel resistance and cell cytotoxicity test were performed. Four defect types in nine rabbit calvaria were randomly allocated: i) control, ii) membrane, iii) deproteinized porcine bone mineral (DPBM) covered by membrane with DOPA, and iv) DPBM covered by membrane with cyanoacrylate. Animals were sacrificed at 2 (n=4) and 8 weeks (n=5) for microcomputed tomography and histomorphometric analysis. DOPA showed low peel resistance but high cell viability. RESULT: Cyanoacrylate and DOPA groups showed significantly higher mineralized tissue volume (MTV) compared to control and membrane groups at 2 weeks (P < 0.05). At 8 weeks, DOPA group showed the highest MTV. Significantly higher new bone area was found in DOPA group at 8 weeks (P < 0.05). Bone formation increased from 2 to 8 weeks in DOPA group (P < 0.05).
CONCLUSION
DOPA showed high cell viability and in vivo study revealed predictable performance in bone regeneration.

Keyword

Adhesive; Bone regeneration; Dihydroxyphenyalanine

MeSH Terms

Adhesiveness
Adhesives*
Animals
Bone Regeneration*
Cell Survival
Collagen*
Cyanoacrylates
Dihydroxyphenylalanine*
Membranes*
Miners*
Osteogenesis
Skull
X-Ray Microtomography
Adhesives
Collagen
Cyanoacrylates
Dihydroxyphenylalanine