Biomol Ther.  2019 Jan;27(1):71-77. 10.4062/biomolther.2018.051.

Neuroprotective Effects of Spinosin on Recovery of Learning and Memory in a Mouse Model of Alzheimer's Disease

• Xu F
• He B
• Xiao F
• Yan T
• Bi K
• Jia Y
• Wang Z

• KMID: 2432069
• DOI: http://doi.org/10.4062/biomolther.2018.051

Abstract

Previous studies have shown that spinosin was implicated in the modulation of sedation and hypnosis, while its effects on learning and memory deficits were rarely reported. The aim of this study is to investigate the effects of spinosin on the improvement of cognitive impairment in model mice with Alzheimer's disease (AD) induced by Aβ1-42 and determine the underlying mechanism. Spontaneous locomotion assessment and Morris water maze test were performed to investigate the impact of spinosin on behavioral activities, and the pathological changes were assayed by biochemical analyses and histological assay. After 7 days of intracerebroventricular (ICV) administration of spinosin (100 µg/kg/day), the cognitive impairment of mice induced by Aβ1-42 was significantly attenuated. Moreover, spinosin treatment effectively decreased the level of malondialdehyde (MDA) and Aβ1-42 accumulation in hippocampus. Aβ1-42 induced alterations in the expression of brain derived neurotrophic factor (BDNF) and B-cell lymphoma-2 (Bcl-2), as well as inflammatory response in brain were also reversed by spinosin treatment. These results indicated that the ameliorating effect of spinosin on cognitive impairment might be mediated through the regulation of oxidative stress, inflammatory process, apoptotic program and neurotrophic factor expression, suggesting that spinosin might be beneficial to treat learning and memory deficits in patients with AD via multi-targets.

Keyword

Spinosin; Semen Ziziphi spinosae; Alzheimer's disease; Neuroprotection

MeSH Terms

Alzheimer Disease*
Animals
B-Lymphocytes
Brain
Brain-Derived Neurotrophic Factor
Cognition Disorders
Hippocampus
Humans
Hypnosis
Learning*
Locomotion
Malondialdehyde
Memory Disorders
Memory*
Mice*
Neuroprotection
Neuroprotective Agents*
Oxidative Stress
Water
Brain-Derived Neurotrophic Factor
Malondialdehyde
Neuroprotective Agents
Water