Yonsei Med J.  2017 Mar;58(2):272-281. 10.3349/ymj.2017.58.2.272.

Comparative Effects of Non-Thermal Atmospheric Pressure Plasma on Migration and Invasion in Oral Squamous Cell Cancer, by Gas Type

  • 1Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea. ostium@ajou.ac.kr
  • 2Department of Otorhinolaryngology, Konyang University College of Medicine, Daejeon, Korea.
  • 3Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
  • 4Department of Electrical and Computer Engineering, Ajou University, Suwon, Korea.
  • 5Department of Life Science, Ajou University, Suwon, Korea.
  • 6Ajou University, Suwon, Korea.
  • 7ICD Co., Ltd., Anseong, Korea.


The fourth state of matter, plasma is known as an ionized gas with electrons, radicals and ions. The use of non-thermal plasma (NTP) in cancer research became possible because of the progresses in plasma medicine. Previous studies on the potential NTP-mediated cancer therapy have mainly concentrated on cancer cell apoptosis. In the present study, we compared the inhibitory effect of NTP on cell migration and invasion in the oral squamous cancer cell lines.
We used oral squamous cancer cell lines (SCC1483, MSKQLL1) and different gases (Nâ‚‚, He, and Ar). To investigate the mechanism of plasma treatment, using different gases (Nâ‚‚, He, and Ar) which induces anti-migration and anti-invasion properties, we performed wound healing assay, invasion assay and gelatin zymography.
The results showed that NTP inhibits cancer cell migration and invasion of oral squamous cancer cell. In addition, focal adhesion kinase expression and matrix metalloproteinase-2/9 activity were also inhibited.
The suppression of cancer cell invasion by NTP varied depending on the type of gas. Comparison of the three gases revealed that Nâ‚‚ NTP inhibited cell migration and invasion most potently via decreased expression of focal adhesion kinase and matrix metalloproteinase activity.


Non thermal atmospheric pressure plasma; oral squamous cancer cell; focal adhesion kinase; matrix metallopeptidase-2/9; paxillin

MeSH Terms

Apoptosis/drug effects
Carcinoma, Squamous Cell/*metabolism
Cell Line, Tumor
Cell Movement/*drug effects
Focal Adhesion Protein-Tyrosine Kinases
Matrix Metalloproteinase 2/genetics/*metabolism
Matrix Metalloproteinase 9/genetics/*metabolism
Mouth Neoplasms/metabolism/*therapy
Neoplasm Invasiveness
Plasma Gases/*pharmacology
Wound Healing
Plasma Gases
Focal Adhesion Protein-Tyrosine Kinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
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