Int Neurourol J.  2018 Oct;22(Suppl 3):S139-S146. 10.5213/inj.1836228.114.

Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice

• Moon EJ
• Ko IG
• Kim SE
• Jin JJ
• Hwang L
• Kim CJ
• An H
• Lee BJ
• Yi JW

• KMID: 2426692
• DOI: http://doi.org/10.5213/inj.1836228.114

Abstract

PURPOSE
Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice.
METHODS
For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed.
RESULTS
Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment.
CONCLUSIONS
In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.

Keyword

Sleep deprivation; Dexmedetomidine; Depression; Serotonin; Dopamine

MeSH Terms

Animals
Blotting, Western
Depression
Dexmedetomidine*
Dopamine
Hindlimb Suspension
Immunohistochemistry
Mice*
Physical Exertion
Receptors, Dopamine
Serotonin
Sleep Deprivation
Tryptophan Hydroxylase
Tyrosine 3-Monooxygenase
Up-Regulation
Water
Dexmedetomidine
Dopamine
Receptors, Dopamine
Serotonin
Tryptophan Hydroxylase
Tyrosine 3-Monooxygenase
Water