J Cancer Prev.  2018 Mar;23(1):37-43. 10.15430/JCP.2018.23.1.37.

β-Carotene Inhibits Activation of NF-κB, Activator Protein-1, and STAT3 and Regulates Abnormal Expression of Some Adipokines in 3T3-L1 Adipocytes

Affiliations
  • 1Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea.
  • 2Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA.
  • 3Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea. kim626@yonsei.ac.kr

Abstract

BACKGROUND
Oxidative stress occurs in white adipose tissue and dysregulates the expression of adipokines secreted from adipocytes. Since adipokines influence inflammation, supplementation with antioxidants might be beneficial for preventing oxidative stress-mediated inflammation in adipocytes and inflammation-associated complications. β-Carotene is the most prominent antioxidant carotenoid and scavenges reactive oxygen species in various tissues. The purpose of this study was to determine whether β-carotene regulates the expression of adipokines, such as adiponectin, monocyte chemoattractant protein-1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES) in 3T3-L1 adipocytes treated with glucose/glucose oxidase (G/GO).
METHODS
3T3-L1 adipocytes were cultured with or without β-carotene and treated with G/GO, which produces H2O2. mRNA and protein levels in the medium were determined by a real-time PCR and an ELISA. DNA binding activities of transcription factors were assessed using an electrophoretic mobility shift assay.
RESULTS
G/GO treatment increased DNA binding affinities of redox-sensitive transcription factors, such as NF-κB, activator protein-1 (AP-1), and STAT3. G/GO treatment reduced the expression of adiponectin and increased the expression of MCP-1 and RANTES. G/GO-induced activations of NF-κB, AP-1, and STAT3 were inhibited by β-carotene. G/GO-induced dysregulation of adiponectin, MCP-1, and RANTES were significantly recovered by treatment with β-carotene.
CONCLUSIONS
β-Carotene inhibits oxidative stress-induced inflammation by suppressing pro-inflammatory adipokines MCP-1 and RANTES, and by enhancing adiponectin in adipocytes. β-Carotene may be beneficial for preventing oxidative stress-mediated inflammation, which is related to adipokine dysfunction.

Keyword

Adipocytes; Adipokines; Beta carotene; Oxidative stress

MeSH Terms

Adipocytes*
Adipokines*
Adiponectin
Adipose Tissue, White
Antioxidants
beta Carotene
Chemokine CCL2
Chemokine CCL5
DNA
Electrophoretic Mobility Shift Assay
Enzyme-Linked Immunosorbent Assay
Inflammation
Oxidative Stress
Oxidoreductases
Reactive Oxygen Species
Real-Time Polymerase Chain Reaction
RNA, Messenger
Transcription Factor AP-1*
Transcription Factors
Adipokines
Adiponectin
Antioxidants
Chemokine CCL2
Chemokine CCL5
DNA
Oxidoreductases
RNA, Messenger
Reactive Oxygen Species
Transcription Factor AP-1
Transcription Factors
beta Carotene
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