J Vet Sci.  2018 Jan;19(1):79-87. 10.4142/jvs.2018.19.1.79.

Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis

Affiliations
  • 1Animal Health Laboratory, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan. higuchi@rakuno.ac.jp
  • 2Department of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
  • 3National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan.

Abstract

Mycoplasma spp. are contagious bacteria, and mycoplasmal mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of Mycoplasma bovis on bMECs has not been fully described. To elucidate the immune response against intramammary infection by M. bovis, we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus, and Escherichia coli on immune-related mRNA expression in bMECs. Transcriptome analysis indicated a significant decrease in the level of mRNA-encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL)-1β, IL-6, tumor necrosis factor alpha, toll-like receptor (TLR) 2, and TLR4 mRNA expression levels were significantly increased in bMECs stimulated with heat-killed M. bovis, but the expression levels were lower than those following stimulation by heat-killed S. aureus or E. coli. Our results suggest that M. bovis weakly affects mRNA expression in bMECs compared to the effects of E. coli or S. aureus. Moreover, live M. bovis may induce suppression of the immune response in bMECs.

Keyword

Mycoplasma bovis; bovine mammary epithelial cells; cytokines; innate immunity; microarray analysis

MeSH Terms

Agriculture
Bacteria
Cytokines
Efficiency
Epithelial Cells*
Escherichia coli
Female
Gene Expression Profiling
Histones
Immunity, Innate*
Interleukin-6
Interleukins
Mastitis
Microarray Analysis
Mycoplasma bovis*
Mycoplasma*
RNA, Messenger
Staphylococcus aureus
Toll-Like Receptors
Tumor Necrosis Factor-alpha
Cytokines
Histones
Interleukin-6
Interleukins
RNA, Messenger
Toll-Like Receptors
Tumor Necrosis Factor-alpha
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