Korean J Physiol Pharmacol.  2017 Sep;21(5):457-464. 10.4196/kjpp.2017.21.5.457.

Effect of etoricoxib on experimental oxidative testicular ischemia-reperfusion damage in rats induced with torsion-detorsion

• Yapanoglu
• Ozkaya F
• Yilmaz AH
• Mammadov R
• Cimen FK
• Hirik E
• Altuner D

• KMID: 2388691
• DOI: http://doi.org/10.4196/kjpp.2017.21.5.457

Abstract

Etoricoxib features antioxidant and anti-inflammatory properties concomitantly, suggesting that it may be beneficial in testicular ischemia reperfusion (I/R) damage. Our aim is to investigate the effects of etoricoxib on testicular I/R damage induced with torsion-detorsion (TD). The etoricoxib + torsion-detorsion (ETD) groups of animals were given etoricoxib in 50 and 100 mg/kg of body weight (ETD-50 and ETD-100), while the testes torsion-detorsion (TTD) and sham operation rat group (SOG) animals were given single oral doses of distilled water as a solvent. TTD, ETD-50 and ETD-100 groups were subjected to 720° degrees torsion for four hours, and detorsion for four hours. The SOG group was not subjected to this procedure. Biochemical, gene expression and histopathological analyses were carried out on the testicular tissues. The levels of malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) were significantly higher, and the levels of total glutathione (tGSH) and glutathione reductase (GSHRd) were significantly lower in the TTD group, compared to the ETD-50, ETD-100 and SOG groups. Etoricoxib at a dose of 100 mg/kg better prevented I/R damage than the 50 mg/kg dose. Etoricoxib may be useful in clinical practice in the reduction of I/R damage on testes caused by torsion-detorsion.

Keyword

Etoricoxib; Gene expression; Ischemia-reperfusion; Torsion-detorsion

MeSH Terms

Animals
Body Weight
Gene Expression
Glutathione
Glutathione Reductase
Interleukin-1beta
Ischemia
Malondialdehyde
Peroxidase
Rats*
Reperfusion
Testis
Tumor Necrosis Factor-alpha
Water
Glutathione
Glutathione Reductase
Interleukin-1beta
Malondialdehyde
Peroxidase
Tumor Necrosis Factor-alpha
Water

Figure

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  Fig. 1 MDA, MPO, tGSH and GSHRd levels in the testis tissue.

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  Fig. 2 The amount of IL-1β and TNF-α levels in the testis tissue.

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  Fig. 3 IL-1β and TNF-α gene expression levels in the testis tissue.

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  Fig. 4 Histological appearance of the testicular tissue in SOG and TTD groups.(A) Normal histological appearance of the testicular tissue in SOG group. The section shows TA-Tunica albuginea, IA-Interstitium, LH-Leydig cell, L-Lumen and stages of germ cells (SG-Spermatogonium, SH-Sertoli cell, PS-Primary spermatid, SD-Spermatid) in the seminiferous tubules (Magnification, ×10; HE&200). (B) Histopathological appearance of TTD control group: the section shows seminiferous tubule destruction (circle-arrow), interstitial tissue damage (line-arrow) and diffuse edema (square-arrow) (Magnification, ×10; HE&200). (C) Histopathological appearance of TTD control group: The section shows seminiferous tubule structure containing coagulative necrosis (arrow) (Magnification, ×10; HE&400). (D) Histopathological appearance of TTD control group: The section shows destructed seminiferous tubule involving diffuse germ cell damage (square-arrow) and diffuse leydig cell damage in the interstitium (circle-arrow) (Magnification, ×10; HE&200). Scale bars, 50 µm.

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  Fig. 5 Histopathological appearance of ETD-50 and ETD-100 groups.(A) Histopathological appearance of ETD-50 group. Dilated congested blood vessel (arrow) in the interstitium (Magnification, ×20; HE&200; Scale bar, 20 µm). (B) Histopathological appearance of ETD-50 group: The section shows mild disorganization and sporadic edema in the seminiferous tubule (arrow) (Magnification, ×20; HE&200; Scale bar, 20 µm). (C) Histopathological appearance of ETD-50 group: focal decreased spermatid in some seminiferous tubules (arrow) (Magnification, ×20; HE&200; Scale bar, 20 µm). (D) Histopathological appearance of ETD-100 group: The section shows dilated congested vessel structure (arrow) (Magnification, ×10; HE&200; Scale bar, 50 µm).

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