Biomol Ther.
2017 May;25(3):315-320. 10.4062/biomolther.2016.069.
Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
- Affiliations
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- 1School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea. sunjinboo@jejunu.ac.kr
- 2School of Medicine No.2, Jeju National University, Jeju 63241, Republic of Korea. sunjinboo@jejunu.ac.kr
- KMID: 2378810
- DOI: http://doi.org/10.4062/biomolther.2016.069
Abstract
- We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
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