Yeungnam Univ J Med.  1990 Dec;7(2):27-37. 10.12701/yujm.1990.7.2.27.

Distribution patterns of cytoskelectal proteins in cardiac endothelial cells : Investigation using monoclonal antibodies

• Kim HC
• Song IH
• Lee YC

• KMID: 2377280
• DOI: http://doi.org/10.12701/yujm.1990.7.2.27

Abstract

To investigate the changing patterns of microfilament and microtubule arrangement and influence of myocardial cells and colchicines to microfilament and microtubule formation in cardiac endothelial cells the authors carried out indirect immunofluorescence stain for actin and tubulin with supernatant monoclonal antibodies. Secondary antibodies were IgG FITC conjugate. The results were summarized as follows. Fiberform reactions were stronger in the cells with many processes and spread cytoplasm and they became weaker after the endothelial cells formed monolayer. In the endothelial cells cocultured with myocardial cells the fiberform of the microtubule became less visible compared to control group but fiberform of the microtubule maintained strong intensity as endothelial cells formed monolayer. In the group treated with colchicines, there were no visible differences in microfilaments compared to control group but fiberform of microtubule revealed weaker intensity after colchicines treatment. The intensity of microtubule fiberform returned to control level after 2 days.

MeSH Terms

Actin Cytoskeleton
Actins
Antibodies
Antibodies, Monoclonal*
Cytoplasm
Endothelial Cells*
Fluorescein-5-isothiocyanate
Fluorescent Antibody Technique, Indirect
Immunoglobulin G
Microtubules
Tubulin
Actins
Antibodies
Antibodies, Monoclonal
Fluorescein-5-isothiocyanate
Immunoglobulin G
Tubulin