Korean J Immunol.  1997 Sep;19(3):391-398.

Production of Monoclonal Antibody Against Human 14 - 3 - 3 Zeta Isoform Expressed in Escherichia coli

• Lim BU
• Kang JS
• Kim JW
• Choi NJ

Abstract

14-3-3 proteins are cytoplasmic proteins of about 29 kDa and have a minimum of seven isoforms. This protein is important in signal transduction with the ability of binding with phosphoserine of many signalling proteins. We expressed 14-3-3 protein tagged with 6 histidine residues in E. coli and purified the protein by nickel affinity chromatography. Using this purified protein as an antigen, we made rabbit antisera and mouse monoclonal antibodies to 14-3-3 zeta isoform. We subcloned cDNA of 14-3-3 zeta isoform derived from HeLa cell lamda gt 11 library into an E. coli expression vector which is designed to express heterologous protein with N- terminal 6 hidtidine tag. BALB/c mice were immunized with purified 14-3-3 protein and the hybridoma clones which produce monoclonal antibodies angainst 14-3-3 protein were selected. These monoclonal antibodies reacted with the recombinant protein expressed in E. coli as well as the 29-kDa native protein in various cell lines. However, they did not immunoprecipitate 14-3-3 protein. The monoclonal antibodies produced in this study can be valuable tools for the identification of the 14-3-3 in signal transduction study.

Keyword

Monoclonal antibody; Expression

MeSH Terms

14-3-3 Proteins
Animals
Antibodies, Monoclonal
Cell Line
Chromatography, Affinity
Clone Cells
Cytoplasm
DNA, Complementary
Escherichia coli*
Escherichia*
HeLa Cells
Histidine
Humans*
Hybridomas
Immune Sera
Mice
Nickel
Phosphoserine
Protein Isoforms
Signal Transduction
Staphylococcal Protein A
14-3-3 Proteins
Antibodies, Monoclonal
DNA, Complementary
Histidine
Immune Sera
Nickel
Phosphoserine
Protein Isoforms
Staphylococcal Protein A