Classification of the Carbohydrate using pectin Binding Characteristics and PAS Reaction in Human Gingiva

Park, Bong Soo; Kim, Jin Jeong; Kim, Jae Bong; Kim, Ju Hee; Yoon, Sik; Kim, Dong Hoan

Abstract

This study was performed in order to recognize the identifications of the glycoproteins containing oligosaccharides in human gingiva. After made paraffin sections of human gingiva at 4µm, the sections were incubated with 7 lectins (UEA-I, BS-I, SBA, DBA, WGA, PNA, PNA after neuraminidase treated, Con-A). In order to increase specificity of reactions, the sections were applicated with ABC system. And then the sections were incubated with DAB and were counterstained with hematoxylin. Using the same sections, the sections were done H-E and PAS stains. In WGA, DBA and Con-A, plasma membranes of the layers of all epithelium and connective tissue were stained. In BS-I ; In the epithelium of marginal gingiva, plasma membranes of upper layer of the spinous cell layer and granular cell layer were stained. And in epithelium of sulcular gingiva, plasma membranes of the all spinous cell layer and granular cell layer were stained. In SBA ; Plasma membranes of the granular cell layer were stained. In PNA ; In the epithelium of marginal gingiva, plasma membranes of the basal cell layer and lower layer of spinous cell layer were stained. But lectin reactions were not occurred in thc sulcular gingiva. In PNA treated neuraminidase, plasma membranes of the all epithelial layer except basal cell layer membranes especially cytoplasms of upper layer at the sulcular gingiva and connective tissue were reacted. 1. By the above results, authors could know the identification of oligosaccharides existing g1ycoproteins in the human gingiva. 1) All epithelial layer ; Î±-D-N-Acetyl-Galactosamine, Sialic acid, D-Glucosamine, Î±-D-Mannose 2) Basal cell layer ; Galactose-Î²-(1-3)-N-Acetyl-Galactosamine 3) Spinous cell layer ; Î±-D-Galactose, Galactose-Î²-(1-3)-N-Acetyl-Galactosamine 4) Granular cell layer ; Î±-D-Galactose 5) Connective tissue ; Î±-D-N-Acetyl-Galactosamine, Siallic acid, Î²-(1-4)-D-Acetyl-Glucosamine, Î±-D-Glucosamine, Î±-D-Mannose 2. The Galactose-Î²-(1-3)-N-Acetyl-Galactosamine was not existed in the basal cell layer and spinous cell layer in the sulcular gingiva.