Biomol Ther.  2017 Mar;25(2):165-170. 10.4062/biomolther.2016.063.

Chemical Constituents Identified from Fruit Body of Cordyceps bassiana and Their Anti-Inflammatory Activity

Affiliations
  • 1School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
  • 2Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. sangheeshim@duksung.ac.kr
  • 3Institute for Bio-Medical Convergence, International St. Mary’s Hospital and College of Medicine, Catholic Kwandong University, Incheon 22711, Republic of Korea.
  • 4College of Pharmacy, Duksung Women’s University, Seoul 01369, Republic of Korea. sangheeshim@duksung.ac.kr

Abstract

Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.

Keyword

Cordyceps bassiana; Phytochemical study; 1,9-dimethylguanine; NF-κB; AP-1; Anti-inflammatory activity

MeSH Terms

Adenosine
Agaricales
Animals
Chromatography
Chromatography, High Pressure Liquid
Cordyceps*
Dermatitis
Dermatitis, Atopic
Fruit*
Genes, Reporter
Luciferases
Mannitol
Mice
Niacinamide
Prostaglandin-Endoperoxide Synthases
RNA, Messenger
Transcription Factor AP-1
Tumor Necrosis Factor-alpha
Uridine
Adenosine
Luciferases
Mannitol
Niacinamide
Prostaglandin-Endoperoxide Synthases
RNA, Messenger
Transcription Factor AP-1
Tumor Necrosis Factor-alpha
Uridine
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