Biomol Ther.
2017 Mar;25(2):165-170. 10.4062/biomolther.2016.063.
Chemical Constituents Identified from Fruit Body of Cordyceps bassiana and Their Anti-Inflammatory Activity
- Affiliations
-
- 1School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
- 2Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. sangheeshim@duksung.ac.kr
- 3Institute for Bio-Medical Convergence, International St. Mary’s Hospital and College of Medicine, Catholic Kwandong University, Incheon 22711, Republic of Korea.
- 4College of Pharmacy, Duksung Women’s University, Seoul 01369, Republic of Korea. sangheeshim@duksung.ac.kr
- KMID: 2375036
- DOI: http://doi.org/10.4062/biomolther.2016.063
Abstract
- Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.
Keyword
MeSH Terms
-
Adenosine
Agaricales
Animals
Chromatography
Chromatography, High Pressure Liquid
Cordyceps*
Dermatitis
Dermatitis, Atopic
Fruit*
Genes, Reporter
Luciferases
Mannitol
Mice
Niacinamide
Prostaglandin-Endoperoxide Synthases
RNA, Messenger
Transcription Factor AP-1
Tumor Necrosis Factor-alpha
Uridine
Adenosine
Luciferases
Mannitol
Niacinamide
Prostaglandin-Endoperoxide Synthases
RNA, Messenger
Transcription Factor AP-1
Tumor Necrosis Factor-alpha
Uridine
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Successful Development of Cordyceps bassiana Stromata from Beauveria bassiana
- Inhibitory Activity of Cordyceps bassiana Extract on LPS-induced Inflammation in RAW 264.7 Cells by Suppressing NF-κB Activation
- Stable Formation of Fruiting Body in Cordyceps bassiana
- Cordyceps bassiana and Production of Stromata in vitro Showing Beauveria Anamorph in Korea
- Cultural Characteristics and Fruiting Body Production in Cordyceps bassiana
